Literature DB >> 2401370

Cloning and sequence analysis of a cDNA for human glycosylasparaginase. A single gene encodes the subunits of this lysosomal amidase.

K J Fisher1, O K Tollersrud, N N Aronson.   

Abstract

We have isolated a full-length cDNA (HPAsn.6) for human placenta glycosylasparaginase using a 221-bp PCR amplified fragment containing rat liver asparaginase gene sequences. The deduced amino acid sequence from the human clone showed sequence identity to both the alpha and beta subunits of the rat enzyme. The human enzyme is encoded as a 34.6 kDa polypeptide that is post-translationally processed to generate two subunits of approx. 19.5 (alpha) and 15 (beta) kDa. A charge enriched region is present at the predicted site where cleavage occurs. Using polyclonal antibodies against the alpha and beta subunits of rat liver asparaginase, we have shown that the human enzyme is similar in structure to the rat enzyme.

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Year:  1990        PMID： 2401370     DOI： 10.1016/0014-5793(90)81211-6

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  16 in total

1.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1991-01-11       Impact factor: 16.971

2.  Comparison of liver glycosylasparaginases from six vertebrates.

Authors:  O K Tollersrud; N N Aronson
Journal:  Biochem J       Date:  1992-03-15       Impact factor: 3.857

3.  Aspartylglycosaminuria in the Finnish population: identification of two point mutations in the heavy chain of glycoasparaginase.

Authors:  I Mononen; N Heisterkamp; V Kaartinen; J C Williams; J R Yates; P R Griffin; L E Hood; J Groffen
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

4.  Single base deletion in exon 7 of the glycosylasparaginase gene causes a mild form of aspartylglycosaminuria in a patient of Mauritian origin.

Authors:  H Park; M Rossiter; A H Fensom; B Winchester; N N Aronson
Journal:  J Inherit Metab Dis       Date:  1996       Impact factor: 4.982

5.  Human aspartylglucosaminidase. A biochemical and immunocytochemical characterization of the enzyme in normal and aspartylglucosaminuria fibroblasts.

Authors:  N Enomaa; T Heiskanen; R Halila; R Sormunen; R Seppälä; M Vihinen; L Peltonen
Journal:  Biochem J       Date:  1992-09-01       Impact factor: 3.857

6.  Purification and structure of human liver aspartylglucosaminidase.

Authors:  J W Rip; M B Coulter-Mackie; C A Rupar; B A Gordon
Journal:  Biochem J       Date:  1992-12-15       Impact factor: 3.857

7.  The isolation and characterisation of a cDNA clone encoding L-asparaginase from developing seeds of lupin (Lupinus arboreus).

Authors:  T J Lough; B D Reddington; M R Grant; D F Hill; P H Reynolds; K J Farnden
Journal:  Plant Mol Biol       Date:  1992-06       Impact factor: 4.076

8.  Characterization of three alleles causing aspartylglycosaminuria: two from a British family and one from an American patient.

Authors:  H Park; M B Vettese; A H Fensom; K J Fisher; N N Aronson
Journal:  Biochem J       Date:  1993-03-15       Impact factor: 3.857

9.  Splicing defect of the glycoasparaginase gene in two Japanese siblings with apartylglucosaminuria.

Authors:  K Yoshida; N Yanagisawa; A Oshima; H Sakuraba; Y Iida; Y Suzuki
Journal:  Hum Genet       Date:  1992 Sep-Oct       Impact factor: 4.132

10.  Chromosomal localization of the human glycoasparaginase gene to 4q32-q33.

Authors:  C Morris; N Heisterkamp; J Groffen; J C Williams; I Mononen
Journal:  Hum Genet       Date:  1992-01       Impact factor: 4.132
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