| Literature DB >> 2401360 |
T Kawamoto1, Y Mitsuuchi, K Toda, K Miyahara, Y Yokoyama, K Nakao, K Hosoda, Y Yamamoto, H Imura, Y Shizuta.
Abstract
A full-length cDNA clone encoding steroid 11 beta-hydroxylase (P-45011 beta) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'-untranslated region and a 576 bp 3'-untranslated region to which a poly(A) tract is attached. The promoter region of the P-450(11) beta gene has also been isolated from a genomic library derived from human pre-B cells. It contains a TATA box, a putative cAMP-responsive element, several repeated sequences and two sequence elements similar to the consensus sequence for binding of AP-1. A transient expression assay in Y-1 adrenal tumor cells demonstrates that the promoter activity is remarkably enhanced by treatment of the cells with cAMP. In addition, analysis using deletion mutants containing various lengths of the 5'-flanking region of the gene suggests that several cis-acting elements participate in transcriptional regulation of human P-450(11) beta gene.Entities:
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Year: 1990 PMID： 2401360 DOI： 10.1016/0014-5793(90)81190-y
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124