Literature DB >> 24011058

Transformation of E. coli via electroporation.

Juliane C Lessard1.   

Abstract

To create electro-competent E. coli and transform them with a plasmid of choice via electroporation.
Copyright © 2013 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Electro-competent E. coli; Electroporation; Escherichia coli; Heat-shock transformation; Plasmid DNA

Mesh：

Year:  2013        PMID： 24011058     DOI： 10.1016/B978-0-12-418687-3.00027-6

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  6 in total

1.  Inactivation of retrotransposon Tos17 Chr.7 in rice cultivar Nipponbare through CRISPR/Cas9-mediated gene editing.

Authors:  Yanchang Luo; Dongsheng Tian; Joanne Chin Yi Teo; Kar Hui Ong; Zhongchao Yin
Journal:  Plant Biotechnol (Tokyo)       Date:  2020-03-25       Impact factor: 1.133

2.  Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria.

Authors:  George M Taylor; Andrew Hitchcock; John T Heap
Journal:  Nucleic Acids Res       Date:  2021-12-02       Impact factor: 16.971

3.  The Discovery of Peptide Macrocycle Rescuers of Pathogenic Protein Misfolding and Aggregation by Integrating SICLOPPS Technology and Ultrahigh-Throughput Screening in Bacteria.

Authors:  Dafni C Delivoria; Georgios Skretas
Journal:  Methods Mol Biol       Date:  2022

4.  Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering.

Authors:  George M Taylor; Paweł M Mordaka; John T Heap
Journal:  Nucleic Acids Res       Date:  2019-02-20       Impact factor: 16.971

5.  Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks.

Authors:  Yuan-Jyue Chen; Sundipta D Rao; Georg Seelig
Journal:  J Vis Exp       Date:  2015-11-25       Impact factor: 1.355

6.  Integrated in Silico and Experimental Approach towards the Design of a Novel Recombinant Protein Containing an Anti-HER2 scFv.

Authors:  Joana Santos; Miguel Cardoso; Irina S Moreira; João Gonçalves; João D G Correia; Sandra Cabo Verde; Rita Melo
Journal:  Int J Mol Sci       Date:  2021-03-29       Impact factor: 5.923
  6 in total

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