| Literature DB >> 24011056 |
Megan Bergkessel1, Christine Guthrie.
Abstract
Colony PCR is a method for rapidly screening colonies of yeast or bacteria that have grown up on selective media following a transformation step, to verify that the desired genetic construct is present, or to amplify a portion of the construct.Entities:
Keywords: Colony PCR; DNA extraction; DNA-intercalating dye; Primer synthesis; Product visualization; Thermostable polymerase
Mesh:
Year: 2013 PMID: 24011056 DOI: 10.1016/B978-0-12-418687-3.00025-2
Source DB: PubMed Journal: Methods Enzymol ISSN: 0076-6879 Impact factor: 1.600