Literature DB >> 24011055

General PCR.

Kirstie Canene-Adams1.   

Abstract

The primary purpose of polymerase chain reaction (PCR) is to rapidly make many copies of a specific region of DNA or RNA so that it can be adequately detected, often by agarose gel electrophoresis. PCR is routinely used to amplify, modify, and clone genes for expression studies. There are many other applications for PCR, including paternity testing, biological relationships, mouse genotyping, diagnosing genetic diseases, forensics, and finding bacteria and viruses.
Copyright © 2013 Elsevier Inc. All rights reserved.

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Keywords:  Agarose gel; Denaturation, annealing, and extension; Phenol-chloroform extraction technique; Polymerase chain reaction (PCR); Thermal cycler, PCR

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Year:  2013        PMID: 24011055     DOI: 10.1016/B978-0-12-418687-3.00024-0

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN: 0076-6879            Impact factor:   1.600


  1 in total

1.  One-Step Genotyping Method in loxP-Based Conditional Knockout Mice Generated by CRISPR-Cas9 Technology.

Authors:  He Zhu; Siqian Liu; Wenxi He; Fei Sun; Yang Li; Ping Yang; Qilin Yu; Shu Zhang
Journal:  Mol Biotechnol       Date:  2022-05-03       Impact factor: 2.860

  1 in total

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