| Literature DB >> 24011046 |
Abstract
The primary purpose of this protocol is to prepare genomic DNA libraries that can then be analyzed by massively parallel next-generation sequencing on the Applied Biosystems SOLiD platform. This protocol can be adapted to next-generation sequencing workflows to ultimately generate up to 1 billion 50 bp sequence tags from the ends of each of the DNA molecules in the library in a single next-generation sequencing run.Entities:
Keywords: Adaptor ligation; Amplification; DNA fragments; End-Repair of fragmented DNA; Integrated DNA Technologies (IDT); Invitrogen E-Gel system; Next-generation sequencing
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Year: 2013 PMID： 24011046 PMCID： PMC3991121 DOI： 10.1016/B978-0-12-418687-3.00015-X
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600