| Literature DB >> 24011040 |
Abstract
Many methods have been developed for the cloning of PCR products. These methods include blunt-end cloning, TA cloning, and using restriction sites incorporated into the PCR primers. The restrictionless cloning technique allows efficient directional cloning of PCR products into any cloning site within a vector regardless of whether the sites are contained within the insert to be cloned.Keywords: Denaturation and annealing; Ligation and transformation; Polymerase chain reaction (PCR); Primer design; Restrictionless cloning; Vector preparation
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Year: 2013 PMID： 24011040 DOI： 10.1016/B978-0-12-418687-3.00009-4
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600