Literature DB >> 24011032

Explanatory chapter: PCR primer design.

Rubén Álvarez-Fernández1.   

Abstract

This chapter is intended as a guide on polymerase chain reaction (PCR) primer design (for information on PCR, see General PCR and Explanatory Chapter: Troubleshooting PCR). In the next section, general guidelines will be provided, followed by a discussion on primer design for specific applications. A list of recommended software tools is shown at the end.
Copyright © 2013 Elsevier Inc. All rights reserved.

Keywords:  Homologous recombination; Multiplex PCR; PCR Primer design; Primer length; Real-time PCR; Reverse-transcription PCR (RT-PCR); Target cloning

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Year:  2013        PMID： 24011032     DOI： 10.1016/B978-0-12-418687-3.00001-X

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  5 in total

1.  Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe.

Authors:  Catarina A Marques; Nicholas J Dickens; Daniel Paape; Samantha J Campbell; Richard McCulloch
Journal:  Genome Biol       Date:  2015-10-19       Impact factor: 13.583

2.  Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays.

Authors:  Yasumasa Kimura; Takahiro Soma; Naoko Kasahara; Diane Delobel; Takeshi Hanami; Yuki Tanaka; Michiel J L de Hoon; Yoshihide Hayashizaki; Kengo Usui; Matthias Harbers
Journal:  PLoS One       Date:  2016-02-10       Impact factor: 3.240

3.  Multiplex PCR for simultaneous genotyping of kdr mutations V410L, V1016I and F1534C in Aedes aegypti (L.).

Authors:  Karina Villanueva-Segura; Gustavo Ponce-Garcia; Beatriz Lopez-Monroy; Esteban Mora-Jasso; Lucia Perales; Francisco J Gonzalez-Santillan; Kevin Ontiveros-Zapata; Jesus A Davila-Barboza; Adriana E Flores
Journal:  Parasit Vectors       Date:  2020-06-25       Impact factor: 3.876

4.  Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation.

Authors:  Catarina A Marques; Calvin Tiengwe; Leandro Lemgruber; Jeziel D Damasceno; Alan Scott; Daniel Paape; Lucio Marcello; Richard McCulloch
Journal:  Nucleic Acids Res       Date:  2016-03-06       Impact factor: 16.971

5.  UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection.

Authors:  Roberto Alcántara; Katherin Peñaranda; Gabriel Mendoza-Rojas; Jose A Nakamoto; Eva Dueñas; Daniela Alvarez; Vanessa Adaui; Pohl Milón
Journal:  STAR Protoc       Date:  2021-09-25
  5 in total

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