Literature DB >> 2400602

Digoxigenin-labeled probes can detect single-copy genes in human metaphase chromosomes.

P Vernole1.   

Abstract

A technique of in situ hybridization on metaphases of chromosomes by a digoxigenin-labeled probe is described. This technique was able to detect single DNA sequences of 2 and 7 kilobases. The results obtained were compared with those of a biotin streptavidin alkaline phosphatase-based detection system. The digoxigenin method was at least as efficient and sensitive as the biotin-streptavidin method.

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Year:  1990        PMID： 2400602

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  3 in total

1.  The intracellular localization of human cytomegalovirus DNA in peripheral blood leukocytes during active infections by high-resolution fluorescence in situ hybridization.

Authors:  H Hackstein; H Kirchner; G Jahn; G Bein
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

2.  Fluorescence in situ hybridization with cosmid clones for the detection of human cytomegalovirus DNA in peripheral blood leukocytes.

Authors:  H Hackstein; G Jahn; H Kirchner; G Bein
Journal:  Histochem Cell Biol       Date:  1996-08       Impact factor: 4.304

3.  Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridization. Expression of mRNA of the seminal vesicle secretion protein II and androgen receptor genes in the rat prostate.

Authors:  P Komminoth; F B Merk; I Leav; H J Wolfe; J Roth
Journal:  Histochemistry       Date:  1992-11
  3 in total

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