Literature DB >> 24003200

Labeling individual neurons in the brains of live Xenopus tadpoles by electroporation of dyes or DNA.

Edward S Ruthazer, Anne Schohl, Neil Schwartz, Aydin Tavakoli, Marc Tremblay, Hollis T Cline.   

Abstract

This protocol describes the targeted introduction of fluorophore in the form of a dye or genetic material into single cells. This method has the advantage of producing true single-cell chimeric animals in which to study the effects of overexpression or knockdown of a gene in an otherwise entirely wild-type background.

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Year:  2013        PMID: 24003200     DOI: 10.1101/pdb.prot077149

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN: 1559-6095


  1 in total

1.  Imaging Structural and Functional Dynamics in Xenopus Neurons.

Authors:  Hollis T Cline
Journal:  Cold Spring Harb Protoc       Date:  2022-02-01
  1 in total

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