OBJECTIVES: To determine the prevalence of antibiotic resistance and the epidemiology of Escherichia coli bacteraemia isolates across the Yorkshire and Humber National Health Service region over 2 years. METHODS: Ten percent of all E. coli blood culture isolates were collected per month from 14 laboratories across the Yorkshire and Humber region. Individual laboratories submitted antibiotic susceptibility data and isolates were re-tested centrally using the VITEK2(®) system (bioMérieux, France). Isolates were also characterized using PCR to test for the presence of sequences encoding extended-spectrum β-lactamases (ESBLs) and genotyped using amplified fragment length polymorphism (AFLP). Selected isolates were further characterized using multilocus sequence typing. RESULTS: Between July 2010 and June 2012, 770 isolates were examined: 63%, 40%, 14% and 7% of isolates were non-susceptible to ampicillin, trimethoprim, ciprofloxacin and gentamicin, respectively. Eight percent of isolates (n = 63) were ESBL positive; CTX-M group 1 enzymes were the most common (68%). There was a fluctuating trend in the prevalence of resistance to amoxicillin/clavulanic acid (MIC >8 mg/L): July-September 2010, 16%; July-September 2011, 38%; and April-June 2012, 22%. AFLP identified 106 types. The majority of isolates belonged to one of two AFLP types: AFLP 1 [sequence type (ST) 131; 17%] and AFLP 2 (ST73; 18%). ST131 and ST73 were both associated with hospital- and community-onset bacteraemia, and with urinary, hepatobiliary and gastrointestinal sources of infection. ESBL-positive isolates were predominantly ST131 (60%). CONCLUSIONS: Continued surveillance of antibiotic resistance among E. coli bacteraemia isolates is necessary to enhance these early baseline data. The variable prevalence of resistance to amoxicillin/clavulanic acid raises concerns, as both E. coli bacteraemia and empirical use of this antibiotic are common.
OBJECTIVES: To determine the prevalence of antibiotic resistance and the epidemiology of Escherichia coli bacteraemia isolates across the Yorkshire and Humber National Health Service region over 2 years. METHODS: Ten percent of all E. coli blood culture isolates were collected per month from 14 laboratories across the Yorkshire and Humber region. Individual laboratories submitted antibiotic susceptibility data and isolates were re-tested centrally using the VITEK2(®) system (bioMérieux, France). Isolates were also characterized using PCR to test for the presence of sequences encoding extended-spectrum β-lactamases (ESBLs) and genotyped using amplified fragment length polymorphism (AFLP). Selected isolates were further characterized using multilocus sequence typing. RESULTS: Between July 2010 and June 2012, 770 isolates were examined: 63%, 40%, 14% and 7% of isolates were non-susceptible to ampicillin, trimethoprim, ciprofloxacin and gentamicin, respectively. Eight percent of isolates (n = 63) were ESBL positive; CTX-M group 1 enzymes were the most common (68%). There was a fluctuating trend in the prevalence of resistance to amoxicillin/clavulanic acid (MIC >8 mg/L): July-September 2010, 16%; July-September 2011, 38%; and April-June 2012, 22%. AFLP identified 106 types. The majority of isolates belonged to one of two AFLP types: AFLP 1 [sequence type (ST) 131; 17%] and AFLP 2 (ST73; 18%). ST131 and ST73 were both associated with hospital- and community-onset bacteraemia, and with urinary, hepatobiliary and gastrointestinal sources of infection. ESBL-positive isolates were predominantly ST131 (60%). CONCLUSIONS: Continued surveillance of antibiotic resistance among E. coli bacteraemia isolates is necessary to enhance these early baseline data. The variable prevalence of resistance to amoxicillin/clavulanic acid raises concerns, as both E. coli bacteraemia and empirical use of this antibiotic are common.
Authors: Ana Paula de Souza da-Silva; Viviane Santos de Sousa; Natacha Martins; Rubens Clayton da Silva Dias; Raquel Regina Bonelli; Lee W Riley; Beatriz Meurer Moreira Journal: Diagn Microbiol Infect Dis Date: 2017-02-07 Impact factor: 2.803
Authors: Amee R Manges; Hyun Min Geum; Alice Guo; Thaddeus J Edens; Chad D Fibke; Johann D D Pitout Journal: Clin Microbiol Rev Date: 2019-06-12 Impact factor: 26.132
Authors: Holly Ciesielczuk; Jonathon Betts; Lynnette Phee; Michel Doumith; Russell Hope; Neil Woodford; David W Wareham Journal: Virulence Date: 2015 Impact factor: 5.882
Authors: M J Day; M Doumith; J Abernethy; R Hope; R Reynolds; J Wain; D M Livermore; N Woodford Journal: J Antimicrob Chemother Date: 2016-05-05 Impact factor: 5.790
Authors: M Doumith; M Day; H Ciesielczuk; R Hope; A Underwood; R Reynolds; J Wain; D M Livermore; N Woodford Journal: J Clin Microbiol Date: 2014-10-29 Impact factor: 5.948
Authors: Tessa E LeCuyer; Barbara A Byrne; Joshua B Daniels; Dubraska V Diaz-Campos; G Kenitra Hammac; Claire B Miller; Thomas E Besser; Margaret A Davis Journal: J Clin Microbiol Date: 2018-08-27 Impact factor: 5.948
Authors: Nathalie L van der Mee-Marquet; Dominique S Blanc; Houssein Gbaguidi-Haore; Sandra Dos Santos Borges; Quentin Viboud; Xavier Bertrand; Roland Quentin Journal: Front Microbiol Date: 2015-06-30 Impact factor: 5.640