Muhabat Adeola Raji1, Wafaa Jamal, Omoh Ojemhen, Vincent O Rotimi. 1. Department of Medical Microbiology and Parasitology, Lagos State University College of Medicine/Lagos State University Teaching Hospital, Ikeja, Nigeria; BT Health Diagnostic Center, LASUTH, Ikeja, Nigeria. Electronic address: adeola68@gmail.com.
Abstract
OBJECTIVES: This study was carried out to determine a point prevalence of drug resistance and extended-spectrum β-lactamase (ESBL) among members of the family Enterobacteriaceae. METHODS: Consecutive clinically significant non-repetitive isolates obtained from both hospitalized patients and outpatients' samples were studied. The isolates were identified using VITEK 2 while susceptibility testing was performed against 16 antibiotics using the E-test strips. Phenotypic production of ESBL was detected by E-test ESBL method. Positive isolates were confirmed by PCR. RESULTS: Of a total of 102 isolates studied, 43 (42.2%) were Escherichia coli and 32 (31.4%) Klebsiella pneumoniae. These isolates demonstrated remarkable high rates of resistance to the β-lactam antibiotics, except the carbapenems and piperacillin-tazobactam. Fifty-two (51%) were resistant to ≥ 3 classes of drugs and 29 (28.4%) to ≥ 5 drugs. Thirty-eight (37.3%) were ESBL producers. Of these, 21 (55.3%) were E. coli and 12 (31.6%) K. pneumoniae. Thus, the overall prevalence of ESBL-producing E. coli was 20.6% and K. pneumoniae 11.8%. CONCLUSIONS: This study showed an alarmingly high prevalence of antibiotic resistance in invasive Enterobacteriaceae isolates and a high prevalence of ESBL producers in the study center. Antibiotic stewardship and other preventive strategies are recommended to reduce the high rate of resistant bacteria in this hospital.
OBJECTIVES: This study was carried out to determine a point prevalence of drug resistance and extended-spectrum β-lactamase (ESBL) among members of the family Enterobacteriaceae. METHODS: Consecutive clinically significant non-repetitive isolates obtained from both hospitalized patients and outpatients' samples were studied. The isolates were identified using VITEK 2 while susceptibility testing was performed against 16 antibiotics using the E-test strips. Phenotypic production of ESBL was detected by E-test ESBL method. Positive isolates were confirmed by PCR. RESULTS: Of a total of 102 isolates studied, 43 (42.2%) were Escherichia coli and 32 (31.4%) Klebsiella pneumoniae. These isolates demonstrated remarkable high rates of resistance to the β-lactam antibiotics, except the carbapenems and piperacillin-tazobactam. Fifty-two (51%) were resistant to ≥ 3 classes of drugs and 29 (28.4%) to ≥ 5 drugs. Thirty-eight (37.3%) were ESBL producers. Of these, 21 (55.3%) were E. coli and 12 (31.6%) K. pneumoniae. Thus, the overall prevalence of ESBL-producing E. coli was 20.6% and K. pneumoniae 11.8%. CONCLUSIONS: This study showed an alarmingly high prevalence of antibiotic resistance in invasive Enterobacteriaceae isolates and a high prevalence of ESBL producers in the study center. Antibiotic stewardship and other preventive strategies are recommended to reduce the high rate of resistant bacteria in this hospital.
Authors: Cécile Okalla Ebongue; Martial Dongmo Tsiazok; Jean Pierre Nda Mefo'o; Guy Pascal Ngaba; Gérard Beyiha; Dieudonné Adiogo Journal: Pan Afr Med J Date: 2015-03-12
Authors: Kabiru O Akinyemi; Rebecca O Abegunrin; Bamidele A Iwalokun; Christopher O Fakorede; Oliwia Makarewicz; Heinrich Neubauer; Mathias W Pletz; Gamal Wareth Journal: Antibiotics (Basel) Date: 2021-02-01