Ning Li1, FangLi Liu2, LianJun Song1, PingAn Zhang1, MingWu Qiao1, QiuYan Zhao1, WenJie Li3. 1. Food Science Technology College of Henan Agricultural University, Zhengzhou 450002, China. 2. College of Public Health, Zhengzhou University, Zhengzhou 450001, China. 3. College of Public Health, Zhengzhou University, Zhengzhou 450001, China. Electronic address: lwj@zzu.edu.cn.
Abstract
OBJECTIVE: To investigate the effects of maternal lead (Pb) exposure on the learning and memory ability and expression of interleukin1-β (IL1-β), tumor necrosis factor (TNF-α) and beta amyloid protein (Aβ) in cerebral cortex of mice offspring. METHODS: Pb exposure initiated from beginning of gestation to weaning. Pb acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.1%, 0.5% and 1% groups, respectively. On the PND21, the learning and memory ability were tested by water maze test and the Pb levels were also determined by graphite furnace atomic absorption spectrometry. The expression of IL1-β, TNF-α and Aβ in cerebral cortex was measured by immunohistochemistry and western blotting. RESULTS: The Pb levels in blood and cerebral cortex of all exposure groups were significantly higher than that of the control group (P<0.05). In water maze test, the performances of 0.5% and 1% groups were worse than that of the control group (P<0.05). The expression of IL1-β, TNF-α and Aβ was increased in Pb exposed groups than that of the control group (P<0.05). CONCLUSIONS: The high expression of IL1-β, TNF-α and Aβ in the cerebral cortex of pups may contribute to the impairment of learning and memory associated with maternal Pb exposure.
OBJECTIVE: To investigate the effects of maternal lead (Pb) exposure on the learning and memory ability and expression of interleukin1-β (IL1-β), tumor necrosis factor (TNF-α) and beta amyloid protein (Aβ) in cerebral cortex of mice offspring. METHODS:Pb exposure initiated from beginning of gestation to weaning. Pb acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.1%, 0.5% and 1% groups, respectively. On the PND21, the learning and memory ability were tested by water maze test and the Pb levels were also determined by graphite furnace atomic absorption spectrometry. The expression of IL1-β, TNF-α and Aβ in cerebral cortex was measured by immunohistochemistry and western blotting. RESULTS: The Pb levels in blood and cerebral cortex of all exposure groups were significantly higher than that of the control group (P<0.05). In water maze test, the performances of 0.5% and 1% groups were worse than that of the control group (P<0.05). The expression of IL1-β, TNF-α and Aβ was increased in Pb exposed groups than that of the control group (P<0.05). CONCLUSIONS: The high expression of IL1-β, TNF-α and Aβ in the cerebral cortex of pups may contribute to the impairment of learning and memory associated with maternal Pb exposure.
Authors: Kelly M Bakulski; Young Ah Seo; Ruby C Hickman; Daniel Brandt; Harita S Vadari; Howard Hu; Sung Kyun Park Journal: J Alzheimers Dis Date: 2020 Impact factor: 4.472
Authors: Karina Chibowska; Jan Korbecki; Izabela Gutowska; Emilia Metryka; Maciej Tarnowski; Marta Goschorska; Katarzyna Barczak; Dariusz Chlubek; Irena Baranowska-Bosiacka Journal: Int J Mol Sci Date: 2020-02-06 Impact factor: 5.923