REASONS FOR PERFORMING STUDY: Mesenchymal stem cells (MSCs) have been used to treat equine tendonitis with promising results; however, little is known about the potential migration of these cells. OBJECTIVES: To assess the possible migration of MSCs from an implantation site in the superficial digital flexor tendon (SDFT) to a lesion in the SDFT of the contralateral limb. STUDY DESIGN: In vivo experimental study. METHODS: Adipose-derived MSCs were isolated from 4 healthy horses. Lesions were induced in the SDFTs of both forelimbs, followed by intralesional implantation of autologous adipose-derived MSCs labelled with nanocrystals into one of the limbs. Flow cytometry of the peripheral blood mononuclear cells and fluorescence microscopy of biopsies of the SDFT lesions were used to search for the labelled cells. RESULTS: Labelled cells were detected among the peripheral blood mononuclear cells in all animals, but labelled cells were present only in the SDFTs that were treated with the intralesional implants. CONCLUSION: Nanocrystals were a valuable in vivo marker of MSCs to be used for tendonitis treatment. Although migration of MSCs to the bloodstream was observed, it was not possible to identify the labelled cells in the untreated tendons.
REASONS FOR PERFORMING STUDY: Mesenchymal stem cells (MSCs) have been used to treat equinetendonitis with promising results; however, little is known about the potential migration of these cells. OBJECTIVES: To assess the possible migration of MSCs from an implantation site in the superficial digital flexor tendon (SDFT) to a lesion in the SDFT of the contralateral limb. STUDY DESIGN: In vivo experimental study. METHODS: Adipose-derived MSCs were isolated from 4 healthy horses. Lesions were induced in the SDFTs of both forelimbs, followed by intralesional implantation of autologous adipose-derived MSCs labelled with nanocrystals into one of the limbs. Flow cytometry of the peripheral blood mononuclear cells and fluorescence microscopy of biopsies of the SDFT lesions were used to search for the labelled cells. RESULTS: Labelled cells were detected among the peripheral blood mononuclear cells in all animals, but labelled cells were present only in the SDFTs that were treated with the intralesional implants. CONCLUSION: Nanocrystals were a valuable in vivo marker of MSCs to be used for tendonitis treatment. Although migration of MSCs to the bloodstream was observed, it was not possible to identify the labelled cells in the untreated tendons.
Authors: Wei Lee Lim; Ling Ling Liau; Min Hwei Ng; Shiplu Roy Chowdhury; Jia Xian Law Journal: Tissue Eng Regen Med Date: 2019-06-26 Impact factor: 4.169
Authors: Jayesh Dudhia; Patricia Becerra; Miguel A Valdés; Francisco Neves; Neil G Hartman; Roger K W Smith Journal: J Vis Exp Date: 2015-12-09 Impact factor: 1.355
Authors: Randolph L Winter; Wen J Seeto; Yuan Tian; Fred J Caldwell; Elizabeth A Lipke; Anne A Wooldridge Journal: BMC Vet Res Date: 2018-08-23 Impact factor: 2.741
Authors: A B Ahrberg; C Horstmeier; D Berner; W Brehm; C Gittel; A Hillmann; C Josten; G Rossi; S Schubert; K Winter; J Burk Journal: BMC Musculoskelet Disord Date: 2018-07-18 Impact factor: 2.362
Authors: Suzanne J K Mund; Daniel J MacPhee; John Campbell; Ali Honaramooz; Bruce Wobeser; Spencer M Barber Journal: Cells Date: 2021-11-01 Impact factor: 6.600
Authors: Drew W Koch; Lauren V Schnabel; Ilene M Ellis; Rowan E Bates; Alix K Berglund Journal: Stem Cell Res Ther Date: 2022-09-16 Impact factor: 8.079
Authors: Florian Geburek; Kathrin Mundle; Sabine Conrad; Maren Hellige; Ulrich Walliser; Hans T M van Schie; René van Weeren; Thomas Skutella; Peter M Stadler Journal: Stem Cell Res Ther Date: 2016-02-01 Impact factor: 6.832
Authors: Danielle Jaqueta Barberini; Monica Aleman; Fabio Aristizabal; Mathieu Spriet; Kaitlin C Clark; Naomi J Walker; Larry D Galuppo; Rogério Martins Amorim; Kevin D Woolard; Dori L Borjesson Journal: Stem Cell Res Ther Date: 2018-04-10 Impact factor: 6.832