AIMS: Lichen sclerosus (LS) is a chronic inflammatory disease of the genital skin of unknown aetiology. The role of LS in penile squamous cell carcinogenesis is not well characterized. HPV has been implicated in both, as have epigenetic changes. The presence of HPV and hypermethylation of the MGMT, p16, RASSF1, RASSF2, TSLC1 and TSP1 genes were studied in penile LS; MGMT, RASSF2 and TSLC1 hypermethylation in penile cancer and TSLC1 hypermethylation in vulvar LS and cancer extends previous results reported by our group. METHODS AND RESULTS: Thirty-seven HPV genotypes and hypermethylation were evaluated by PCR/reverse-line-blot and methylation-specific PCR respectively, in 27 preputial LS, 24 penile SCC, 30 vulvar SCC, 21 vulvar LS and 22 normal skin cases. HPV66 was present in 3.7% of penile LS cases, and p16 and RASSF2 hypermethylation were more frequent in penile cancer than in penile LS. p16, RASSF1, RASSF2 and TSP1 hypermethylation were similar in penile and vulvar LS. CONCLUSIONS: Gene hypermethylation is a common event in penile LS, and occurs approximately as frequently as in vulvar LS. Certain genes can be hypermethylated as an early or late event in LS or cancer, respectively. This suggests a possible sequential role for these alterations in the transition from benign to malignant lesions.
AIMS: Lichen sclerosus (LS) is a chronic inflammatory disease of the genital skin of unknown aetiology. The role of LS in penile squamous cell carcinogenesis is not well characterized. HPV has been implicated in both, as have epigenetic changes. The presence of HPV and hypermethylation of the MGMT, p16, RASSF1, RASSF2, TSLC1 and TSP1 genes were studied in penile LS; MGMT, RASSF2 and TSLC1 hypermethylation in penile cancer and TSLC1 hypermethylation in vulvar LS and cancer extends previous results reported by our group. METHODS AND RESULTS: Thirty-seven HPV genotypes and hypermethylation were evaluated by PCR/reverse-line-blot and methylation-specific PCR respectively, in 27 preputial LS, 24 penile SCC, 30 vulvar SCC, 21 vulvar LS and 22 normal skin cases. HPV66 was present in 3.7% of penile LS cases, and p16 and RASSF2 hypermethylation were more frequent in penile cancer than in penile LS. p16, RASSF1, RASSF2 and TSP1 hypermethylation were similar in penile and vulvar LS. CONCLUSIONS: Gene hypermethylation is a common event in penile LS, and occurs approximately as frequently as in vulvar LS. Certain genes can be hypermethylated as an early or late event in LS or cancer, respectively. This suggests a possible sequential role for these alterations in the transition from benign to malignant lesions.
Authors: E Martín-Sánchez; E Pernaut-Leza; S Mendaza; A Cordoba; F Vicente-Garcia; I Monreal-Santesteban; J Pérez Vizcaino; M J Díaz De Cerio; N Perez-Janices; I Blanco-Luquin; D Escors; A Ulazia-Garmendia; D Guerrero-Setas Journal: Virchows Arch Date: 2016-04-21 Impact factor: 4.064
Authors: N Perez-Janices; I Blanco-Luquin; M T Tuñón; E Barba-Ramos; B Ibáñez; I Zazpe-Cenoz; M Martinez-Aguillo; B Hernandez; E Martínez-Lopez; A F Fernández; M R Mercado; T Cabada; D Escors; D Megias; D Guerrero-Setas Journal: Oncotarget Date: 2015-01-01
Authors: Nikki B Thuijs; Johannes Berkhof; Müjde Özer; Sylvia Duin; Annina P van Splunter; Barbara C Snoek; Daniëlle A M Heideman; Marc van Beurden; Renske D M Steenbergen; Maaike C G Bleeker Journal: Int J Cancer Date: 2021-01-10 Impact factor: 7.396
Authors: Noemi Perez-Janices; Idoia Blanco-Luquin; Natalia Torrea; Therese Liechtenstein; David Escors; Alicia Cordoba; Francisco Vicente-Garcia; Isabel Jauregui; Susana De La Cruz; José Juan Illarramendi; Valle Coca; Maria Berdasco; Grazyna Kochan; Berta Ibañez; José Miguel Lera; David Guerrero-Setas Journal: Oncotarget Date: 2015-09-15