Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells.

OBJECTIVE(S): Sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. The present study was designed to analyze the effect of Sertoli cells in differentiation of mouse embryonic stem cells (mESCs) to germ cells.
MATERIALS AND METHODS: A fusion construct composed of a Stra8 gene promoter and the coding region of enhanced green fluorescence protein was produced to select differentiated mESCs. To analyze sertoli cells' effect in differentiation process, mESCs were separated into two groups: the first group was cultured on gelatin with retinoic acid treatment and the second group was co-cultured with sertoli cell feeder without retinoic acid induction. Expressions of pre-meiotic (Stra8), meiotic (Dazl and Sycp3) and post-meiotic (Prm1) genes were evaluated at different differentiation stages (+7, +12 and +18 days of culture).
RESULTS: In the first group, expressions of meiotic and post-meiotic genes started 12 and 18 days after induction with retinoic acid, respectively. In the second group, 7 days after co-culturing with Sertoli cells, expression of meiotic and post-meiotic genes was observed.
CONCLUSION: These results show that differentiation process to germ cells is supported by Sertoli cells. Our findings provide a novel effective approach for generation of germ cell in vitro and studying the interaction of germ cells with their niche.

Introduction

An estimated 10 to 15 percent of couples worldwide have fertility problems, half of whom being infertile men (1). The causes of male infertility are numerous. One of the untreatable causes is sertoli-cell-only (SCO) syndrome which is characterized by azoospermia and lack of germ cells in testis biopsy (2). Recent studies have shown that mouse embryonic stem cells (mESCs) are able to give rise to primordial germ cells (PGCs) and sperm-like cells in vitro (3). mESCs are derived from inner cell mass and have two properties: self renewal and pluripotency (4). The ability to generate germ cells from mESCs provides a powerful in vitro model to study germ cell development and offers new therapeutic approaches to infertility (5).

Spermatogenesis is a complex process in which undifferentiated germ cells undergoes successive mitotic and meiotic divisions and a morphological reorganization, in order to produce a cell with capability of fertilizing an oocyte. The process of mammalian spermatogenesis can be divided into two phases: 1) prenatal phase consists of the migration of primordial germ cells (PGC) from yolk sac to gonadal ridges and testicular organogenesis and 2) a postnatal phase in which spermatogonial stem cells differentiate into mature spermatozoa. In prenatal phase, PGCs (spermatogonial precursors) and Sertoli cell precursors aggregate and form the testicular cords (6).

Spermatogenesis is influenced by many factors, such as hormones (endocrine and paracrine), cell-cell interaction, surrounding microenvironment and many other factors. One of the most important factors is Sertoli cells whose role in supporting spermatogonial stem cells has been proved. It has been shown that Sertoli-germ cell communication guarantees the production of healthy sperms (7). In a recent study, it has been shown that conditioned medium from sertoli cell contain some factors that could induce in vitro germ cell differentiation (8).

Deleted in Azoospermia-Like (Dazl) gene regulates germ cell development. Dazl is expressed during spermatogenesis in spermatogonia and primary spermatocytes and it seems to play a key role in meiosis initiation (5). Synaptonemal complex (SC) is a meiotic-specific structure. There are three SC component proteins in mammals (SCP1, SCP2 and SCP3). SCP3 (known as Sycp3) is supposed to constitute the core of these proteins (9). Finally, Prm1 which codes for protamines, is a post-meiotic gene with specific expression in haploid round spermatids (10).

The purpose of the present study was to compare the process of in vitro differentiation of mESCs to germ cell in two different conditions: regular culture system and co-culture on Sertoli cells as feeder layer.

Materials and Methods

Mouse embryonic stem cell line C57BL6 with normal male (XY) karyotype (Invitrogen) was cultured in an undifferentiated state on a feeder layer of mitomycin C-inactivated mouse embryonic fibroblasts (11). Culture medium composed of Knockout Dulbecco’s modified Eagle’s medium (Knockout DMEM, GIBCO-BRL) was supplemented with 12.5% (v/v) ES qualified FBS (GIBCO-BRL), 2 mmol L-Glutamine (GIBCO-BRL), 1X nonessential amino acids (NEAA; GIBCO-BRL), 50 µgml-1 penicillin and streptomycin, 50 µmol β-mercaptoethanol and 10³ unit ml-1 LIF (Milipore). Mouse Sertoli cell line was then cultured in the medium composed of Ham'S F12 and Dulbecco’s modified Eagle’s medium (DMEM), 2.5 % (v/v) Fetal bovine serum (FBS, GIBCO-BRL) and 5% (v/v) Horse serum (Sigma).

A segment of Stra8 (Stimulated by Retinoic Acid 8) gene (-1400/+7) was amplified from genomic DNA and inserted in the SacI/HindIII site of modified pEGFP-1 vector, where neomycin-resistance cassette was replaced with puromycin-resistance gene (12). ES cells were trypsinized and around 7×106 cells were prepared for performing electroporation. Linearized vector (35 µg) was electroporated into ES cells. Electroporation was performed on Bio-Rad GenePulser device at 250 V and 500 µF. After electroporation, mESCs were transferred into six wells plate and cultured for 72 hr without antibiotic. Puromycin (final concentration 1 µg ml-1) was added to the medium and cells were cultured in the presence of puromycin for three weeks. Subsequently, puromycin resistant colonies were selected. DNA extraction was performed from these colonies tested for presence of Stra8/EGFP vector by PCR. Positive colonies were grown in a complete medium with LIF for a month.

Retinoic acid (RA) was added to the medium at a final concentration of 10ˉ5 mol for 72 hr to induce differentiation. GFP-positive cells were then selected using fluorescence-activated cell sorting (FACS). Cells were trypsinized and after pipetting up and down few times filtered through a 40 μm strainer (Falcon) to create single-cell suspensions. Sorting was carried out with 3×106 cells using BD FACS Aria II flow cytometer (13). These cells were cultured on an MEF feeder under non-induced condition for two weeks. Resulting colonies were divided into two groups for comparing the process of differentiation in different conditions. Group 1 was cultured for 18 days on gelatin with RA treatment (final concentration 10-8 mol) and group 2 was co-cultured on mouse Sertoli cell line (TM4) without RA treatment (Figure 1). The culture media of both groups was the same (except for RA).

Figure 1

Co-culture of mESCs (arrows) colonies and Sertoli cells

Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol. RNA concentration was measured with Nano Drop 1000 spectrophotometer (Thermo Fisher Scientific). The extracted RNA (2 µg) was employed for cDNA synthesis using M-MLV reverse transcriptase (Fermentase) with random hexamer and oligo dT primer together. RT-PCR was performed using a specific primer for EGFP, Oct4, Stra8, Sycp3, Dazl and Prm1genes (Table 1).

Table 1

r sequence and expected size of PCR products of Stra8, EGFP, Dazl, Sycp3 and protamine-1 genes

Gene	Primer sequence	Product size (bp)
Stra8	F : ACAACCTAAGGAAGGCAGTTTACR: TGACCTCCTCTAAGCTGTTGG	174
EGFP	F: GCACCATCTTCTTCAAGGACGACR: TCTTTGCTCAGGGCGGACTG	270
Dazl	F: CAGGCATATCCTCCTTATCCAAGR: TGTATGCTTCGGTCCACAGAC	263
Sycp3	F: CCGGAGCCGCTGAGCAAACAR: CCAGTTCCCACTGCTGCAACAC	430
Prm1	F: CTCACAGGTTGGCTGGCTCGACR: CGGCGACGGCAGCATCTTCG	192

Results

In order to isolate differentiated stem cells, a specific reporter construct consisting of a germ line-specific segment of Stra8 gene promoter and the coding region of enhanced green fluorescence (EGFP) protein were used. The use of this 1.4 Kb Stra8 promoter caused testis-specific expression of GFP. After selection with puromycin, ES colonies were chosen and PCR with GFP specific primers was Dazl, Sycp3 and Prm1 were not detected in induced and non-induced cells. Moreover, Stra8 is a molecular marker for spermatogonial stem cells (13); therefore it was concluded that induced cells were spermatogonial stem cell (SSC)-like cells.

Diagram of GFP expressed mESCs sorted by FACS. b and c: Sorted cells showed high expression of GFP

In this study, SSC-like cells were cultured in two groups and mRNA of these cells was extracted in periods of 7, 12 and 18 days of culture (Figure 3). These samples were analyzed for pre-meiotic (Oct4 and Stra8), meiotic (Sycp3 and Dazl), and post- meiotic (Prm1) genes expression. In both groups, expression of Oct4, Stra8, and Dazl were observed on expression was started on the 7th day in the second group. There was no expression of Prm1 until the 7th day of cell culture. In the first group, Sycp3 was expressed on the 12th day of culture, though the 18th day in the first group. However, the expression of Prm1 started on the 7th day of culture in group 2 (Table 2). As a control, the expression of these genes was negative in the TM4 Sertoli cell line (data not shown).

Figure 3

Expression of meiotic (Sycp3 and Dazl) and postmeiotic (Prm1) genes in two groups of culture: Group 1: culture of mESCs on gelatin with RA treatment,Group 2: co-cultured of mESCs with Sertoli cell feeder without RA induction

Table 2

Expression pattern of Oct4, Stra8, Dazl, Sycp3 and Prm1 genes in two groups: Group 1: culture of mESCs on gelatin with RA treatment, Group 2: culture of mESCs on Sertoli cells without RA induction

		Oct4	Stra8	Dazl	Sycp3	Prm1
Undifferentiated mESCs		+	−	−	−	−
after 72 hr induction with RA		+	+	−	−	−
Group 1	Day 7	+	+	+	−	−
	Day 12	+	+	+	+	−
	Day 18	+	+	+	+	+
Group 2	Day 7	+	+	+	+	+

Discussion

This study provides an in vitro model of spermatogenesis in which the effects of cell-cell interaction and microenvironment may be examined.

During transition of mammalian germ cells from mitosis to meiosis Stra8 gene is expressed (14). A vector composed of promoter region of Stra8 gene and GFP coding sequence was used in this study to select stable transfected ES cells that entered meiosis. We observed that if we GFP-positive cells were not separated, which entered meiosis stage in the cell culture; differentiation process could be suppressed by neighboring undifferentiated cell populations. This finding has also been observed previously (15) and that is why FACS was used for cellular separation.

Dazl is a testis-specific gene with a critical role in the spermatogenesis. Recent studies have shown that Dazl is a key factor in meiosis initiation (16, 17). Deletion of Dazl gene in knockout mice, results in meiosis arrest which may reduce the expression of some specific genes involved in sperm production (5). In this study, detection of Dazl expression showed that SSC-like cells has been entered meiosis in both groups (with and without Sertoli cells) after seven days of culture. Sycp3 is a meiotic specific gene responsible for the formation of synaptonemal complex. This complex has an important role in synapses formation, recombination and segregation of chromosomes during meiosis (9).

Histones were replaced by Protamin1, a post-meiotic gene, in sperm chromatin (15). The difference in expression pattern of Sycp3 and Prm1 genes in two groups may be due to the Sertoli cells role in the process of differentiation of mESCs to germ cells.

Sertoli cells are located within the seminiferous tubules and support spermatogenesis through cell-cell contact (18). It has been shown that Sertoli cells are the main site of RA production in the testis. Sertoli cells provide RA for germ cells in two different ways: by direct delivery of RA to germ cells and delivery of retinol via membrane receptor, STAR6 (19). These findings support our results that Sertoli cells provide some necessary factors for in vitro differentiation of mESCs. Co-culture of ESC and Sertoli cells will promote differentiation process, number and diameter of colonies compared to the culture of ESC in gelatin. These results will be supported by further studies whit using the co-culture of extracted germ and Sertoli cells from human testis (20).

Conclusion

Differentiation is a complex process which requires many differentiating signals and Sertoli cells secret germ cell supporting factors such as RA. This model of in vitro germ cell production may open a new window to understand the effect of other factors on germ cell differentiation.