Literature DB >> 23997341

Detection of rotavirus from hospitalized diarrheic children in uttar pradesh, India.

S K Dash1, Krishna Kumar, A Tewari, P Varshney, A Goel, A K Bhatia.   

Abstract

In the present study 220 stool samples collected from diarrheic children admitted to different hospitals and nursing homes of Uttar Pradesh and Uttarakhand were screened for rotavirus. Of 220 diarrheic samples screened 46 samples were found to be positive for rotavirus by RNA PAGE. All the isolates exhibited 4-2-3-2 migration pattern suggesting group A rotavirus. Both long and short electropherotypes were prevalent in these regions. Six different electropherotypes were detected in this study period. Male diarrheic children were found to be more susceptible to rotavirus infection (22.96 %) than that of the female ones (17.64 %). Viral RNA isolated from stool samples again subjected to VP4 gene amplification by RT-PCR using con2 and con3 primer which resulted 876 bp product suggesting group A rotavirus. Besides virus isolation was successfully done using MA104 cell line.

Entities:  

Keywords:  Cell culture; Epidemiological studies; Human rotavirus; RNA-polyacrylamide gel electrophoresis (RNA-PAGE); RT-PCR

Year:  2012        PMID： 23997341      PMCID： PMC3460112          DOI： 10.1007/s12088-012-0279-6

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN： 0046-8991            Impact factor:   2.461


  28 in total

1.  Comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group A rotaviruses.

Authors:  M Iturriza-Gomara; J Green; D W Brown; U Desselberger; J J Gray
Journal:  J Virol Methods       Date:  1999-03       Impact factor: 2.014

2.  [Cultivation and serial propagation of a new rotavirus causing adult diarrhea in primary human embryo kidney cells].

Authors:  Shaozhong Ji; Ye Bi; Hongyan Yang; Fengrong Yang; Junqi Song; Xiaoxia Tao; Xiaoying Cui
Journal:  Zhonghua Yi Xue Za Zhi       Date:  2002-01-10

3.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

4.  Molecular epidemiology of human rotavirus infections in Chandigarh (India).

Authors:  V Singh; S Broor; S Mehta; S K Mehta
Journal:  Indian J Med Res       Date:  1990-01       Impact factor: 2.375

5.  Sequence analysis of gene 11 equivalents from "short" and "super short" strains of rotavirus.

Authors:  S M Matsui; E R Mackow; S Matsuno; P S Paul; H B Greenberg
Journal:  J Virol       Date:  1990-01       Impact factor: 5.103

6.  Epidemiological profile of rotaviral infection in India: challenges for the 21st century.

Authors:  Gagandeep Kang; Shobhana D Kelkar; Shoba D Chitambar; Pratima Ray; Trailokyanath Naik
Journal:  J Infect Dis       Date:  2005-09-01       Impact factor: 5.226

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 8.  The molecular epidemiology of rotavirus gastroenteritis.

Authors:  M K Estes; D Y Graham; D H Dimitrov
Journal:  Prog Med Virol       Date:  1984

9.  Isolation of human rotavirus subgroups 1 and 2 in cell culture.

Authors:  T Kutsuzawa; T Konno; H Suzuki; A Z Kapikian; T Ebina; N Ishida
Journal:  J Clin Microbiol       Date:  1982-10       Impact factor: 5.948

10.  Rotavirus infection among children in Bombay.

Authors:  H S Desai; D D Banker
Journal:  Indian J Med Sci       Date:  1993-02
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.