BACKGROUND AND SIGNIFICANCE: There is a need to develop non-invasive diagnostic tools to achieve early and accurate detection of skin cancer in a non-surgical manner. In this study, we evaluate the capability of stimulated Raman scattering (SRS) microscopy, a potentially non-invasive optical imaging technique, for identifying the pathological features of s squamous cell carcinoma (SCC) tissue. STUDY DESIGN: We studied ex vivo SCC and healthy skin tissues using SRS microscopy, and compared the SRS contrast with the contrast obtained in reflectance confocal microscopy (RCM) and standard histology. RESULTS AND CONCLUSION: SRS images obtained at the carbon-hydrogen stretching vibration at 2945 cm(-1) exhibit contrast related protein density that clearly delineates the cell nucleus from the cell cytoplasm. The morphological features of SCC tumor seen in the SRS images show excellent correlation with the diagnostic features identified by histological examination. Additionally, SRS exhibits enhanced cellular contrast in comparison to that seen in confocal microscopy. In conclusion, SRS represents an attractive approach for generating protein density maps with contrast that closely resembles histopathological contrast of SCC in human skin.
BACKGROUND AND SIGNIFICANCE: There is a need to develop non-invasive diagnostic tools to achieve early and accurate detection of skin cancer in a non-surgical manner. In this study, we evaluate the capability of stimulated Raman scattering (SRS) microscopy, a potentially non-invasive optical imaging technique, for identifying the pathological features of s squamous cell carcinoma (SCC) tissue. STUDY DESIGN: We studied ex vivo SCC and healthy skin tissues using SRS microscopy, and compared the SRS contrast with the contrast obtained in reflectance confocal microscopy (RCM) and standard histology. RESULTS AND CONCLUSION: SRS images obtained at the carbon-hydrogen stretching vibration at 2945 cm(-1) exhibit contrast related protein density that clearly delineates the cell nucleus from the cell cytoplasm. The morphological features of SCC tumor seen in the SRS images show excellent correlation with the diagnostic features identified by histological examination. Additionally, SRS exhibits enhanced cellular contrast in comparison to that seen in confocal microscopy. In conclusion, SRS represents an attractive approach for generating protein density maps with contrast that closely resembles histopathological contrast of SCC in human skin.
Authors: Chad A Lieber; Shovan K Majumder; Dean Billheimer; Darrel L Ellis; Anita Mahadevan-Jansen Journal: J Biomed Opt Date: 2008 Mar-Apr Impact factor: 3.170
Authors: Melissa C Skala; Jayne M Squirrell; Kristin M Vrotsos; Jens C Eickhoff; Annette Gendron-Fitzpatrick; Kevin W Eliceiri; Nirmala Ramanujam Journal: Cancer Res Date: 2005-02-15 Impact factor: 12.701
Authors: Ralph Peter Braun; Harold S Rabinovitz; Margaret Oliviero; Alfred W Kopf; Jean-Hilaire Saurat Journal: J Am Acad Dermatol Date: 2005-01 Impact factor: 11.527
Authors: Christian W Freudiger; Wei Min; Brian G Saar; Sijia Lu; Gary R Holtom; Chengwei He; Jason C Tsai; Jing X Kang; X Sunney Xie Journal: Science Date: 2008-12-19 Impact factor: 47.728
Authors: J K Barton; K W Gossage; W Xu; J R Ranger-Moore; K Saboda; C A Brooks; L D Duckett; S J Salasche; J A Warneke; D S Alberts Journal: Technol Cancer Res Treat Date: 2003-12
Authors: Fa-Ke Lu; Srinjan Basu; Vivien Igras; Mai P Hoang; Minbiao Ji; Dan Fu; Gary R Holtom; Victor A Neel; Christian W Freudiger; David E Fisher; X Sunney Xie Journal: Proc Natl Acad Sci U S A Date: 2015-08-31 Impact factor: 11.205
Authors: Rebecca C Hoesli; Daniel A Orringer; Jonathan B McHugh; Matthew E Spector Journal: Otolaryngol Head Neck Surg Date: 2017-04-11 Impact factor: 3.497