Literature DB >> 23996188

Single channel analysis of membrane proteins in artificial bilayer membranes.

Philipp Bartsch1, Anke Harsman, Richard Wagner.   

Abstract

The planar lipid bilayer technique is a powerful experimental approach for electrical single channel recordings of pore-forming membrane proteins in a chemically well-defined and easily modifiable environment. Here we provide a general survey of the basic materials and procedures required to set up a robust bilayer system and perform electrophysiological single channel recordings of reconstituted proteins suitable for the in-depth characterization of their functional properties.

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Year:  2013        PMID: 23996188     DOI: 10.1007/978-1-62703-487-6_22

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  5 in total

1.  tRNAs and proteins use the same import channel for translocation across the mitochondrial outer membrane of trypanosomes.

Authors:  Moritz Niemann; Anke Harsman; Jan Mani; Christian D Peikert; Silke Oeljeklaus; Bettina Warscheid; Richard Wagner; André Schneider
Journal:  Proc Natl Acad Sci U S A       Date:  2017-08-28       Impact factor: 11.205

2.  Oep23 forms an ion channel in the chloroplast outer envelope.

Authors:  Tom Alexander Goetze; Manali Patil; Ingrid Jeshen; Bettina Bölter; Sabine Grahl; Jürgen Soll
Journal:  BMC Plant Biol       Date:  2015-02-12       Impact factor: 4.215

3.  Molecular architecture of black widow spider neurotoxins.

Authors:  Minghao Chen; Daniel Blum; Lena Engelhard; Stefan Raunser; Richard Wagner; Christos Gatsogiannis
Journal:  Nat Commun       Date:  2021-11-29       Impact factor: 14.919

4.  Reconstitution and functional characterization of ion channels from nanodiscs in lipid bilayers.

Authors:  Laura-Marie Winterstein; Kerri Kukovetz; Oliver Rauh; Daniel L Turman; Christian Braun; Anna Moroni; Indra Schroeder; Gerhard Thiel
Journal:  J Gen Physiol       Date:  2018-02-27       Impact factor: 4.086

5.  Silicon Nitride-Based Micro-Apertures Coated with Parylene for the Investigation of Pore Proteins Fused in Free-Standing Lipid Bilayers.

Authors:  Tanzir Ahmed; Jayesh Arun Bafna; Roland Hemmler; Karsten Gall; Richard Wagner; Mathias Winterhalter; Michael J Vellekoop; Sander van den Driesche
Journal:  Membranes (Basel)       Date:  2022-03-09
  5 in total

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