Marc Bardou1, Tarik Hadi1, Guillaume Mace2, Matthieu Pesant3, Julie Debermont4, Marina Barrichon5, Maeva Wendremaire6, Nicole Laurent7, Paul Sagot8, Frédéric Lirussi6. 1. Centre d'Investigations Cliniques Plurithématique 803 (INSERM CIC-P 803), Dijon, France; Center for Lipid Research, INSERM (Institut National de la Santé et de la Recherche Médicale), Dijon, France; CHU de Dijon, Dijon, France. 2. Service de Gynécologie et d'Obstétrique, Dijon, France. 3. Humanitas Clinical and Research Center, Leukocyte Biology Laboratory, University of Milan, Milan, Italy. 4. Centre d'Investigations Cliniques Plurithématique 803 (INSERM CIC-P 803), Dijon, France. 5. Center for Lipid Research, INSERM (Institut National de la Santé et de la Recherche Médicale), Dijon, France; CHU de Dijon, Dijon, France. 6. Center for Lipid Research, INSERM (Institut National de la Santé et de la Recherche Médicale), Dijon, France; CHU de Dijon, Dijon, France; Laboratoire de Pharmacologie-Toxicologie, Dijon, France. 7. Service d'Anatomie Pathologique, Dijon, France. 8. CHU de Dijon, Dijon, France; Service de Gynécologie et d'Obstétrique, Dijon, France.
Abstract
OBJECTIVES: To study the influence of pregnancy and labor on the proportion and level of activation of monocyte subpopulations in human pregnancy. STUDY DESIGN: Peripheral blood samples were obtained from healthy nonpregnant women (n = 6); women in the third-trimester of healthy pregnancies (n = 18) and women with preterm premature rupture of membranes (n = 46), just before delivery for the last 2 groups. Monocyte subpopulations were characterized by flow cytometry using CD14, CD16, and activation level using macrophage chemoattractant protein-1 (MCP-1) and CCR2 antibodies. RESULTS: The relative proportion of each monocyte subset in nonpregnant women was similar to that in women with healthy or complicated pregnancies. However, pregnancy was associated with a significant decrease in MCP-1 expressing monocytes (79.5% ± 19.8% vs 9.3% ± 6.8% and 11.9% ± 8.3% for nonpregnant, healthy pregnancy, and preterm premature rupture of membranes (respectively, P < .05). Spontaneous labor was associated with a return to nonpregnant values for the proportion of MCP-1 expressing monocytes in both normal (74.4% ± 16.9) and preterm premature rupture of membranes pregnancy (68.4% ± 35.6), irrespective of the mode of delivery (vaginal or cesarean section). This was not observed in women who delivered without spontaneous labor onset. CCR-2 (MCP-1 receptor) expression was not modified in monocytes at the time of labor, but was significantly increased in granulocytes (3646 ± 1080 vs 7338 ± 2718 for nonlaboring and laboring preterm premature rupture of membranes, respectively, P < .05) CONCLUSION: In light of previous reports of a role for MCP-1 in labor, our results suggest the downregulation of activation levels of monocytes, via MCP-1 expression might be involved in maternofetal immune tolerance. Monocyte reactivation might be associated with labor.
OBJECTIVES: To study the influence of pregnancy and labor on the proportion and level of activation of monocyte subpopulations in human pregnancy. STUDY DESIGN: Peripheral blood samples were obtained from healthy nonpregnant women (n = 6); women in the third-trimester of healthy pregnancies (n = 18) and women with preterm premature rupture of membranes (n = 46), just before delivery for the last 2 groups. Monocyte subpopulations were characterized by flow cytometry using CD14, CD16, and activation level using macrophage chemoattractant protein-1 (MCP-1) and CCR2 antibodies. RESULTS: The relative proportion of each monocyte subset in nonpregnant women was similar to that in women with healthy or complicated pregnancies. However, pregnancy was associated with a significant decrease in MCP-1 expressing monocytes (79.5% ± 19.8% vs 9.3% ± 6.8% and 11.9% ± 8.3% for nonpregnant, healthy pregnancy, and preterm premature rupture of membranes (respectively, P < .05). Spontaneous labor was associated with a return to nonpregnant values for the proportion of MCP-1 expressing monocytes in both normal (74.4% ± 16.9) and preterm premature rupture of membranes pregnancy (68.4% ± 35.6), irrespective of the mode of delivery (vaginal or cesarean section). This was not observed in women who delivered without spontaneous labor onset. CCR-2 (MCP-1 receptor) expression was not modified in monocytes at the time of labor, but was significantly increased in granulocytes (3646 ± 1080 vs 7338 ± 2718 for nonlaboring and laboring preterm premature rupture of membranes, respectively, P < .05) CONCLUSION: In light of previous reports of a role for MCP-1 in labor, our results suggest the downregulation of activation levels of monocytes, via MCP-1 expression might be involved in maternofetal immune tolerance. Monocyte reactivation might be associated with labor.
Authors: Brice Gaudillière; Edward A Ganio; Martha Tingle; Hope L Lancero; Gabriela K Fragiadakis; Quentin J Baca; Nima Aghaeepour; Ronald J Wong; Cele Quaintance; Yasser Y El-Sayed; Gary M Shaw; David B Lewis; David K Stevenson; Garry P Nolan; Martin S Angst Journal: Cytometry A Date: 2015-07-17 Impact factor: 4.355
Authors: Nardhy Gomez-Lopez; Roberto Romero; Sonia S Hassan; Gaurav Bhatti; Stanley M Berry; Juan Pedro Kusanovic; Percy Pacora; Adi L Tarca Journal: Front Immunol Date: 2019-12-17 Impact factor: 7.561