OBJECTIVE: To determine whether the presence of soluble human leukocyte antigen G (sHLA-G) affects implantation and pregnancy outcomes in vitro. DESIGN: A multicenter retrospective study. SETTING: Six certified in vitro fertilization (IVF) units. PATIENT(S): Embryos obtained from 2,040 patients from six different IVF clinics. INTERVENTION(S): Soluble HLA-G determination on day-2 embryos after intracytoplasmic sperm injection, with embryos transferred on day 3 using the sHLA-G data. MAIN OUTCOME MEASURE(S): Ongoing pregnancy rate (10- to 12-week ultrasound finding). RESULT(S): All embryos were individually cultured, and a chemiluminescence enzyme-linked immunosorbent assay was used to detect the presence of sHLA-G in the culture medium surrounding the embryos. Embryos were selected based on a positive sHLA-G result and a graduated embryo scoring (GES) score >70, or on embryo morphology if the test was negative. In all centers, a positive sHLA-G result was associated with an increase in the odds of an ongoing pregnancy. The incidence of an ongoing pregnancy was 2.52 times greater in embryos transferred on day 3 with a positive sHLA-G test result than the incidence of an ongoing pregnancy in embryos with a negative sHLA-G test result. CONCLUSION(S): Data from this multicenter study confirm that sHLA-G expression is a valuable noninvasive embryo marker to assist in improving pregnancy outcomes, with the theoretical potential to reduce multiple pregnancies.
OBJECTIVE: To determine whether the presence of soluble human leukocyte antigen G (sHLA-G) affects implantation and pregnancy outcomes in vitro. DESIGN: A multicenter retrospective study. SETTING: Six certified in vitro fertilization (IVF) units. PATIENT(S): Embryos obtained from 2,040 patients from six different IVF clinics. INTERVENTION(S): Soluble HLA-G determination on day-2 embryos after intracytoplasmic sperm injection, with embryos transferred on day 3 using the sHLA-G data. MAIN OUTCOME MEASURE(S): Ongoing pregnancy rate (10- to 12-week ultrasound finding). RESULT(S): All embryos were individually cultured, and a chemiluminescence enzyme-linked immunosorbent assay was used to detect the presence of sHLA-G in the culture medium surrounding the embryos. Embryos were selected based on a positive sHLA-G result and a graduated embryo scoring (GES) score >70, or on embryo morphology if the test was negative. In all centers, a positive sHLA-G result was associated with an increase in the odds of an ongoing pregnancy. The incidence of an ongoing pregnancy was 2.52 times greater in embryos transferred on day 3 with a positive sHLA-G test result than the incidence of an ongoing pregnancy in embryos with a negative sHLA-G test result. CONCLUSION(S): Data from this multicenter study confirm that sHLA-G expression is a valuable noninvasive embryo marker to assist in improving pregnancy outcomes, with the theoretical potential to reduce multiple pregnancies.
Authors: Izabela Nowak; Karolina Wilczyńska; Jacek R Wilczyński; Andrzej Malinowski; Paweł Radwan; Michał Radwan; Piotr Kuśnierczyk Journal: Arch Immunol Ther Exp (Warsz) Date: 2017-05-18 Impact factor: 4.291
Authors: Izabela Nowak; Karolina Wilczyńska; Paweł Radwan; Andrzej Wiśniewski; Rafał Krasiński; Michał Radwan; Jacek R Wilczyński; Andrzej Malinowski; Piotr Kuśnierczyk Journal: Front Immunol Date: 2020-01-14 Impact factor: 7.561
Authors: Roberta Rizzo; Giuseppe Lo Monte; Daria Bortolotti; Angela Graziano; Valentina Gentili; Dario Di Luca; Roberto Marci Journal: Int J Mol Sci Date: 2015-03-10 Impact factor: 5.923