Yuan Yuan1, Songyi Lin1, Na Guo1, Chengcheng Zhao2, Suxia Shen1, Xiujuan Bu1, Haiqing Ye3. 1. Department of Food Quality and Safety, College of Agriculture, Jilin University, Changchun, China. 2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China. 3. Department of Food Quality and Safety, College of Agriculture, Jilin University, Changchun, China. Electronic address: yehq@jlu.edu.cn.
Abstract
BACKGROUND AIMS: Staphylococci account for a large proportion of hospital-acquired infections, especially among patients with indwelling devices. These infections are often caused by biofilm-producing strains, which are difficult to eradicate and may eventually cause bacteremia and metastatic infections. Recent evidence suggests that mesenchymal stem cells can enhance bacterial clearance in vivo. METHODS: In this study, a rat model with carboxymethyl cellulose pouch infection was used to analyze the efficacy of bone marrow-derived mesenchymal stromal cells (BMSCs) against the methicillin-resistant Staphylococcus aureus. RESULTS: The results showed that the administration of BMSCs effectively reduced the number of bacterial colonies and the expression of many cytokines and chemokines (such as interleukin [IL]-6, IL-1β, IL-10 and CCL5). Unlike the fibroblast control groups, the pouch tissues from the BMSC-treated rats showed the formation of granulations, suggesting that the healing of the wound was in progress. CONCLUSIONS: The results indicate that the treatment of BMSCs can reduce methicillin-resistant S aureus infection in vivo, thereby reducing the inflammatory response.
BACKGROUND AIMS: Staphylococci account for a large proportion of hospital-acquired infections, especially among patients with indwelling devices. These infections are often caused by biofilm-producing strains, which are difficult to eradicate and may eventually cause bacteremia and metastatic infections. Recent evidence suggests that mesenchymal stem cells can enhance bacterial clearance in vivo. METHODS: In this study, a rat model with carboxymethyl cellulose pouch infection was used to analyze the efficacy of bone marrow-derived mesenchymal stromal cells (BMSCs) against the methicillin-resistant Staphylococcus aureus. RESULTS: The results showed that the administration of BMSCs effectively reduced the number of bacterial colonies and the expression of many cytokines and chemokines (such as interleukin [IL]-6, IL-1β, IL-10 and CCL5). Unlike the fibroblast control groups, the pouch tissues from the BMSC-treated rats showed the formation of granulations, suggesting that the healing of the wound was in progress. CONCLUSIONS: The results indicate that the treatment of BMSCs can reduce methicillin-resistant S aureus infection in vivo, thereby reducing the inflammatory response.
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