BACKGROUND/AIMS: Acetaminophen (APAP) effects on intestinal barrier properties are less investigated. APAP may lead to a changed bioavailability of a subsequently administered drug or diet in the body. We investigated the influence of APAP on enterocytic cell membrane properties that are able to modify the net intestinal absorption of administered substances across the Caco-2 barrier model. METHODS: The effect of APAP on cytotoxicity was measured by LDH assay, TER value and cell capacitance label-free using impedance monitoring, membrane permeability by FITC-dextrans, and efflux transporter MDR1 activity by Rh123. APAP levels were determined by HPLC analysis. Cell membrane topography and microvilli were investigated using SEM and intestinal alkaline phosphatase (Alpi) and tight junction protein 1 (TJP1) expression by western blot analysis. RESULTS: APAP changed the apical cell surface, reduced the number of microvilli and protein expression of Alpi as a brush border marker and TJP1, increased the membrane integrity and concurrently decreased cell capacitance over time. In addition, APAP decreased the permeability to small molecules and increased the efflux transporter activity, MDR1. CONCLUSION: APAP alters the Caco-2 cell membrane properties by different mechanisms and reduces the permeability to administered substances. These findings may help to optimize therapeutic implications.
BACKGROUND/AIMS: Acetaminophen (APAP) effects on intestinal barrier properties are less investigated. APAP may lead to a changed bioavailability of a subsequently administered drug or diet in the body. We investigated the influence of APAP on enterocytic cell membrane properties that are able to modify the net intestinal absorption of administered substances across the Caco-2 barrier model. METHODS: The effect of APAP on cytotoxicity was measured by LDH assay, TER value and cell capacitance label-free using impedance monitoring, membrane permeability by FITC-dextrans, and efflux transporter MDR1 activity by Rh123. APAP levels were determined by HPLC analysis. Cell membrane topography and microvilli were investigated using SEM and intestinal alkaline phosphatase (Alpi) and tight junction protein 1 (TJP1) expression by western blot analysis. RESULTS:APAP changed the apical cell surface, reduced the number of microvilli and protein expression of Alpi as a brush border marker and TJP1, increased the membrane integrity and concurrently decreased cell capacitance over time. In addition, APAP decreased the permeability to small molecules and increased the efflux transporter activity, MDR1. CONCLUSION:APAP alters the Caco-2 cell membrane properties by different mechanisms and reduces the permeability to administered substances. These findings may help to optimize therapeutic implications.
Authors: Ahmed Ghallab; Reham Hassan; Ute Hofmann; Adrian Friebel; Zaynab Hobloss; Lisa Brackhagen; Brigitte Begher-Tibbe; Maiju Myllys; Joerg Reinders; Nina Overbeck; Selahaddin Sezgin; Sebastian Zühlke; Abdel-Latif Seddek; Walaa Murad; Tim Brecklinghaus; Franziska Kappenberg; Jörg Rahnenführer; Daniela González; Christopher Goldring; Ian M Copple; Rosemarie Marchan; Thomas Longerich; Mihael Vucur; Tom Luedde; Stephan Urban; Ali Canbay; Thomas Schreiter; Michael Trauner; Jephte Y Akakpo; Mojtaba Olyaee; Steven C Curry; Jan-Peter Sowa; Hartmut Jaeschke; Stefan Hoehme; Jan G Hengstler Journal: J Hepatol Date: 2022-02-05 Impact factor: 30.083
Authors: Daniel M Chopyk; Johnasha D Stuart; Matthew G Zimmerman; Jing Wen; Sanjeev Gumber; Mehul S Suthar; Manoj Thapa; Mark J Czaja; Arash Grakoui Journal: Hepatol Commun Date: 2019-07-15