| Literature DB >> 23987365 |
Xiaona Zhao1, Yuanliang Hu, Deyun Wang, Jianzhu Liu, Liwei Guo.
Abstract
Based on our previous research, four sulfated polysaccharide (sPSs) from Tremella and Condonpsis pilosula, sTPStp, sTPS70c, sCPPStp and sCPPS50c, were prepared and their effects on splenic lymphocytes proliferation in vitro and the immune response of ND vaccine in chicken were compared taking the unmodified polysaccharide (uPS) TPStp as control. The results showed that four sPSs could significantly or numerically stimulate splenic lymphocyte proliferation singly or synergistically with LPS in vitro, sTPS70c and sCPPStp demonstrated better effect; promote peripheral lymphocytes proliferation and enhance serum HI antibody titer in chickens vaccinated with ND vaccine, the actions of sPSs were stronger than that of uPS, and sTPS70c at medium dosage presented the best efficacy. These indicated that sulfation modification could improve the immune-enhancing activity of TPS and CPPS, sTPS70c possessed the strongest activity and would be expected as a component of new-type immunopotentiator.Entities:
Keywords: 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide; Antibody titer; CMF-PBS; CSA; Condonpsis pilosula polysaccharide (CPPS); DMSO; DS; LPS; Lymphocyte proliferation; MTT; Pyr; Sulfation modification; TPS; Tremella polysaccharide (TPS); calcium and magnesium-free phosphate-buffered saline; chlorsulfonic acid; degree of sulfation; dimethyl sulfoxide; lipopolysaccharide; pyridine; sCPPSs; sPS; sTPSs; sulfated condonpsis pilosula polysaccharide; sulfated polysaccharide; sulfated tremella polysaccharide; tremella polysaccharide; uPS; unmodified polysaccharide
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Year: 2013 PMID： 23987365 DOI： 10.1016/j.carbpol.2013.06.043
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381