Literature DB >> 23981867

Eliminating false positives in a qPCR assay for the detection of the uidA gene in Escherichia coli.

Richard Kibbee1, Natalie Linklater, Banu Ormeci.   

Abstract

Due to contaminant Escherichia coli DNA present in recombinant Taq polymerase reagents, it is not possible to reliably detect low levels of E. coli in samples using the quantitative polymerase chain reaction (qPCR) assay. Native Taq polymerase was successfully used in this study to detect five uidA gene copies (5 fg of genomic DNA) of the uidA gene.

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Year:  2013        PMID： 23981867     DOI： 10.2166/wh.2013.201

Source DB:  PubMed          Journal:  J Water Health        ISSN： 1477-8920            Impact factor:   1.744


  4 in total

1.  Antibiotic resistance and phylogenetic profiling of Escherichia coli from dairy farm soils; organic versus conventional systems.

Authors:  Omega Y Amoafo; Vanita Malekar; Eirian Jones; Stephen L W On
Journal:  Curr Res Microb Sci       Date:  2021-12-10

2.  Simultaneous detection of viable Salmonella spp., Escherichia coli, and Staphylococcus aureus in bird's nest, donkey-hide gelatin, and wolfberry using PMA with multiplex real-time quantitative PCR.

Authors:  Taobo Liang; Hui Long; Zhongxu Zhan; Yingfei Zhu; Peilin Kuang; Ni Mo; Yuping Wang; Shenghui Cui; Xin Wu
Journal:  Food Sci Nutr       Date:  2022-05-17       Impact factor: 3.553

3.  Chicken Skin Decontamination of Thermotolerant Campylobacter spp. and Hygiene Indicator Escherichia coli Assessed by Viability Real-Time PCR.

Authors:  Imke F Wulsten; Maja Thieck; André Göhler; Elisabeth Schuh; Kerstin Stingl
Journal:  Pathogens       Date:  2022-06-18

Review 4.  Relationship among Shigella spp. and enteroinvasive Escherichia coli (EIEC) and their differentiation.

Authors:  Abu Ud-Din; Syeda Wahid
Journal:  Braz J Microbiol       Date:  2015-03-04       Impact factor: 2.476
  4 in total

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