N-Palmitoylethanolamine depot injection increased its tissue levels and those of other acylethanolamide lipids.

N-Palmitoylethanolamine (NAE 16:0) is an endogenous lipid signaling molecule that has limited water solubility, and its action is short-lived due to its rapid metabolism. This poses a problem for use in vivo as oral administration requires a high concentration for significant levels to reach target tissues, and injection of the compound in a dimethyl sulfoxide- or ethanol-based vehicle is usually not desirable during long-term treatment. A depot injection of NAE 16:0 was successfully emulsified in sterile corn oil (10 mg/kg) and administered in young DBA/2 mice in order to elevate baseline levels of NAE 16:0 in target tissues. NAE 16:0 levels were increased in various tissues, particularly in the retina, 24 and 48 hours following injections. Increases ranged between 22% and 215% (above basal levels) in blood serum, heart, brain, and retina and induced an entourage effect by increasing levels of other 18 carbon N-Acylethanolamines (NAEs), which ranged between 31% and 117% above baseline. These results indicate that NAE 16:0 can be used as a depot preparation, avoiding the use of inadequate vehicles, and can provide the basis for designing tissue-specific dosing regimens for therapies involving NAEs and related compounds.

Introduction

N-Acylethanolamines (NAEs) are endogenous lipid signaling molecules involved in numerous physiological functions in mammals, including neurotransmission and cellular protection.1 NAEs, including anandamide (NAE 20:4), an endogenous ligand of cannabinoid receptor 1 (CB1), are substrates of the fatty acid amide hydrolase (FAAH) enzyme.2,3N-Palmitoylethanolamine (NAE 16:0) is an NAE that has been shown to protect cells from oxidative stress4,5 and activate neuroprotective kinase signaling pathways,4 reduce myocardial infarct volume and neurological behavioral deficits in ischemic rats,6 provide substantial relief of objective and subjective symptoms of atopic eczema,7 decrease melanoma progression,8 and is reported to have anti-inflammatory properties.9 During in vivo studies, NAE 16:0 is rapidly metabolized by FAAH and, therefore, the action of NAE 16:0 is fairly short-lived,1 which poses a problem when administered orally. NAE 16:0 is a fatty acid amide which has very limited water solubility1,9–11 and this is a major issue for in vitro and in vivo treatments, with dimethyl sulfoxide or ethanol vehicle required to dissolve NAE 16:0 at a rather low concentration of 20–25 mM. Often, then, the NAE 16:0 will precipitate out of solution when diluting into media or buffer if the final concentration is not low enough. This makes it difficult to determine a range for higher concentrations of NAE 16:0 in vitro or in vivo. Saline vehicles containing products such as polyethylene glycol, Tween 80,12 or cyclodextrin in combination with ethanol13 have also been used in order to aid solubility for single-dose in vivo administration of NAE 16:0, but NAE 16:0 has not yet been studied as a long-term dosing regimen.

Corn oil is commonly used as a vehicle to administer lipophilic, water-insoluble agents in vivo via gavage feeding, subcutaneous, intraperitoneal, or intramuscular injections.14 Oil-based depot injections are usually performed as an intramuscular or subcutaneous injection in order to achieve a slow and steady release of the compound. Radd et al performed release rate studies of haloperidol in corn oil and several other oils to test for an appropriate vehicle for depot injections (using fatty acids as solubilizers).15 Corn oil was shown to release haloperidol at a rate of 0.113 mg/cm2/h1/2 and was one of the slower releasing oils that were tested.15 Numerous studies use sterile corn oil for injection administration of highly lipophilic compounds, eg, steroid hormones, such as progesterone,16,17 and estradiol,16,18–20 and drugs such as tamoxifen,16,18–26 an estrogen receptor antagonist. The present study examines the effectiveness of NAE 16:0 as a depot injection, which allows for emulsification of the compound at a higher concentration in a corn oil preparation, to avoid precipitation problems. Furthermore, the depot injection of emulsified NAE 16:0 may represent a “slow-release” preparation for future experiments to study the effects of long-term treatments with lipid-soluble acylethanolamides on disease progression in mouse models of disease. For this study we have used young DBA/2 mice (Charles River Laboratories International, Inc, Wilmington, MA, USA), a mouse model of glaucoma, which are nonglaucomatous, to quantify baseline levels of NAE 16:0 in target tissues, and to measure the levels of NAE 16:0 levels in these tissues 24 and 48 hours after depot injections.

In this study, we show that basal levels of NAE 16:0 differ markedly in different tissues of young, non-glaucomatous DBA/2 mice and are particularly elevated in the retina. We also show that NAE 16:0 can be successfully emulsified into an oil depot injection for in vivo administration; the compound effectively reached the blood serum, heart, brain, and retina, and also induced an elevated entourage effect by increasing other acylethanolamide lipids, namely NAE 18:0, 18:1, 18:2, and 20:4.

Materials and methods

Animals

Ten 6-week-old male DBA/2 mice (16–21 g) were obtained from Charles River Laboratories and the mice were housed with five mice per cage. All animals had unlimited access to food and water and were maintained on a 12 hour light/dark cycle. Animals were monitored on a daily basis with no reports of any weight or behavioral changes or pain or distress from the 24–48 hour treatment. All animal husbandry and experimental procedures were conducted in conformity with the Public Health Service Policy on Humane Care and Use of Laboratory Animals, performed in accordance with institutional guidelines and approved by the Institutional Animal Care and Use Committee (University of Missouri – Kansas City Kansas City MO, USA).

NAE 16:0 treatment

Directly before treatment, NAE 16:0 compound (Best West Laboratories Inc, Salt Lake City, UT, USA) was ground using a mortar and pestle and emulsified in sterile corn oil by vortexing and ultrasonification at 37°C for 30 minutes. DBA/2 mice received a 100 μL subcutaneous injection of either sterile corn oil (vehicle, n = 4) or NAE 16:0 (10 mg/kg, based on a previous study6) and were sacrificed by CO2 asphyxiation at either 24 (n = 3) or 48 (n = 3) hours following injections. Blood serum was collected heart, brain, and retina were dissected snap-frozen in liquid nitrogen and stored at −80°C in preparation for lipid extraction.

Lipid extraction and NAE quantification

Lipid extraction was as described previously6 Briefly, the individual tissue samples were weighed (fresh weight [FW]) and homogenized in 2 mL hot 2-propanol (70°C) and internal standards (50 ng each of D4-NAE 16:0 and D4-NAE 20:4 [Cayman Chemical Co, Ann Arbor, MI, USA]) were added. Samples were then incubated in a 70°C water bath for 30 minutes, following which, 1 mL of chloroform was added to each test tube and samples were vortexed and incubated at 4°C for overnight extraction. Monophasic lipid extracts were partitioned with 2 mL of 1 M KCl. The lower organic phase was washed three additional times with 1 M KCl and subsequently dried to completion under argon. The total lipid mass (LM) was estimated gravimetrically and the samples were dissolved in chloroform and stored under argon at −80°C until further purification by solid phase extraction (SPE). Silica SPE cartridges (100 mg, 1.5 mL [Supelco Analytical, Bellefonte, PA, USA]) were conditioned with 2 mL methanol, followed by 4 mL chloroform. Samples dissolved in 1 mL of chloroform were loaded onto SPE cartridges, followed by washing with 2 mL chloroform. NAEs were eluted with 2 mL 1:1 (v/v) ethyl acetate:acetone, evaporated under nitrogen and derivatized in N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA; Thermo Fisher Scientific, Waltham, MA, USA) for 30 minutes at 55°C (12). Derivatized samples were dried under nitrogen and redissolved in 50 μL N-hexane. TMS-ether derivatives of NAEs were identified via selective ion monitoring and quantified against internal standards (D4-NAE 16:0 and D4-NAE 20:4) by gas chromatography/mass spectrometry (model 7890 GC coupled with a 5975C mass selective detector [Agilent Technologies, Santa Clara, CA, USA]).27

Statistical analysis

Data was statistically analyzed using a one-way analysis of variance (ANOVA) with a Dunnett’s or Bonferroni posttest when comparing within treatment groups or control data, respectively. Data are shown as mean ± standard error of the mean.

Results

The highest basal levels of NAE 16:0 (nmol/g FW) were in the retina compared to blood serum (P < 0.001), heart (P < 0.001), and brain (P < 0.001; Figure 1A). The same was true for metabolic data, that basal levels of NAE 16:0 (nmol/g LM) were highest in the retina compared to blood serum (P < 0.01), heart (P < 0.05), and brain (P < 0.001; Figure 1B). Brain had the lowest levels of NAE 16:0 per g FW and LM, when compared to retina, heart (P < 0.05), and blood serum (P < 0.01; Figure 1).

Figure 1

Comparison of control levels of NAE 16:0 in various tissues of DBA/2 mice.

Notes: (A) Functional or (B) metabolic effect of depot injections 24 and 48 hours following administration, showing NAE 16:0 levels per g FW or g LM, respectively. Data within treatment groups or comparing control levels (bars) were statistically analyzed using a one-way ANOVA with a Dunnett’s or Bonferroni posttest, respectively. Data represented as Mean ± standard error of the mean, n = 3–4. *P < 0.05, **P < 0.01, ***P < 0.001.

Abbreviations: FW, fresh weight; LM, lipid mass; NAE 16:0, N-Palmitoyl-ethanolamine.

NAE 16:0 levels were increased significantly over baseline in heart on a tissue weight basis after 24 hours (P < 0.05; Figure 1A) and metabolically on a lipid weight basis after 48 hours (P < 0.001; Figure 1B). NAE 16:0 per g brain tissue showed a sustained increase over 24 and 48 hours (P < 0.05 and FW, P < 0.05, respectively; Figure 1A) and was increased per LM after 24 hours (Figure 1B). NAE 16:0 per g LM in retina significantly increased over baseline after 24 hours (P < 0.01; Figure 1B).

NAE 18:0, 18:1, 18:2, and 20:4 were also increased in some tissues following the NAE 16:0 depot injection (Figure 2). Levels of NAE 18:0 in blood serum (P < 0.001), heart (P < 0.01), brain (P < 0.05), and retina (P < 0.01) were raised after 24 hours (Figure 2) and sustained in heart after 48 hours (P < 0.05; Figure 2B). Levels of 18:1 in blood serum (P < 0.01) and retina (P < 0.05) were elevated after 24 hours and levels in the retina further increased after 48 hours (P < 0.01; Figure 2A and D). Levels of 18:2 were above detection levels in heart and retina only. Levels of 18:2 in retina were significantly raised after 24 hours (P < 0.05; Figure 2D). NAE 20:4 levels in brain (P < 0.05) and retina (P < 0.05) were increased after 24 hours and the retina sustained increased levels over 48 hours (P < 0.05; Figure 2C and D).

Figure 2

Elevation above control levels of NAE 18:0, 18:1, 18:2, and 20:4, 24 and 48 hours following NAE 16:0 depot injection.

Notes: Depot injection in (A) blood serum, (B) heart (C) brain, and (D) retina of DBA/2 mice. Data were statistically analyzed using a one-way analysis of variance (ANOVA) with a Dunnett’s post test. *P < 0.05, **P < 0.01. Mean ± standard error of the mean, n = 3–4.

Abbreviations: FW, fresh weight; NAE, N-Acylethanolamine; NAE 16:0, N-Palmitoylethanolamine.

Discussion

NAEs are lipid signaling molecules that are present in the central nervous system (CNS). The present study measures the levels of NAEs in blood serum, heart, brain, and retina of DBA/2 mice in the absence or presence of NAE 16:0, administered as an oil-based depot injection.

DBA/2 mice showed higher levels of all NAEs in blood serum when compared to other mouse strains and species.1,28–30 Levels of NAE 16:0 and 18:1 were higher in the DBA/2 heart when compared to other mouse strains,31,32 however, lower levels of NAE 20:4 were measured. Brain levels of all NAEs were lower in the DBA/2 mice when compared to other mouse strains and species.31,33 Very low basal levels of NAE 18:2 have been reported in the brain at ~2 pmol/g FW34 and were not detected in this study. NAE 16:0 was much higher in the retina of DBA/2 mice compared to human retina,35,36 but NAE 20:4 had similar retina levels to humans.35,36 These variations in values could be due to differences in species or mouse strain, but are also likely due to differences in extraction and detection measures between various studies.

NAE 16:0 was increased above baseline in serum and heart by 61% and 119% after 24 hours, respectively, and, after 48 hours, NAE 16:0 was increased by 215% per total LM in heart tissue. Per LM, NAE 16:0 levels were significantly increased by 106% and 77% in brain and retina after 24 hours, respectively, and a 63%–65% and a 22%–25% increase was sustained over a 48 hour period per g F W, respectively. Additionally, we showed that the NAE 16:0 depot injection induced an entourage effect of other NAEs. Levels of NAE 18:0 were significantly increased by 45%–115% in all tissues after 24 hours, and the heart sustained increased NAE 18:0 levels of 40%–45% over the 48 hour period. NAE 18:1 was increased in blood serum and retina after 24 hours by 117% and 38%, respectively, and levels were further increased by 20% in the retina 48 hours following the depot injection. NAE 18:2 was only detected in heart and retina, and levels were unchanged in heart but increased by 31% in retina after 24 hours. NAE 20:4 was significantly increased in brain after 24 hours, and the retina sustained a 33%–43% increase over 48 hours.

NAE 20:4 and other polyunsaturated NAEs exert biological activity by binding to cannabinoid receptors (CB).37 CB1 is found distributed throughout the CNS and various tissue38 whereas CB2 is mainly found in the immune system, retina, and CNS.39 NAE 20:4 is also a full agonist for the vanilloid receptor 1 (VR1), an ionotropic cation channel expressed in the peripheral sensory system as well as in the CNS, and acts as nociceptor transducer.40 NAEs with 18 carbon atom acyl chains (NAE 18:0, 18:1, and 18:2) are thought to be potential modulators of VR1.41–43 There is some discussion that NAE 16:0 may act directly on an unidentified CB receptor or be a positive allosteric modulator of the VR1 receptor,40,44 or that NAE 16:0-mediated neuroprotection is thought to act by reducing apoptotic and inflammatory pathways, independent of CB and VR1 activation.37,45,46 NAE 16:0 is thought to be an ‘entourage’ compound for NAE 20:4, which enhances its biological actions, possibly by competing with NAE 20:4 for FAAH, thereby reducing anandamide hydrolysis and increasing anandamide levels consequently, increasing CB receptor activation,44 or by increasing the affinity of NAE 20:4 for receptors.44 Long-term treatment with NAE 16:0, in vitro, was shown to downregulate FAAH.40 Therefore, it is possible that long-term NAE 16:0 administration could simulate the downregulation of FAAH, thus increasing endogenous NAEs, including NAE 20:4, to elicit protection from neurodegeneration in chronic diseases, as well as exerting neuroprotective effects through apoptotic and inflammatory pathways.

Conclusion

This study has shown that NAE 16:0 can be successfully emulsified into an oil depot injection for in vivo administration, eliminating the use of gavage feeding and allowing for a safer vehicle to be administered in vivo, by bypassing the solubility difficulties with NAE 16:0 and eliminating the use of vehicles such as ethanol and dimethyl sulfoxide, which are inadequate for long-term studies. Also, depot injections allow for fewer injections and a steady slow-release of the compound, which can be maintained if studying long-term NAE 16:0 dosing and its effect over time. We also have shown that NAE 16:0, administered as a subcutaneous depot injection, effectively reaches the blood serum, heart, brain, and retina and can induce an elevation in the tissue content of other NAEs. Overall, this study allows for the long-term administration of NAE 16:0, which can induce elevated levels of other NAEs, either by inhibiting the FAAH enzyme or via alternative routes, for in vivo experiments targeting the protection of tissues from acute or chronic degeneration such as in neuroprotection.4,39