Literature DB >> 23976816

Kinetics of CO Recombination to the Heme in Geobacillus Stearothermophilus Nitric Oxide Synthase.

Charlotte A Whited1, Jeffrey J Warren, Katherine D Lavoie, Jay R Winkler, Harry B Gray.   

Abstract

We report the kinetics of CO rebinding to the heme in His134Ser, Ile223Val and His134Ser/Ile223Ser mutants of Geobacillus stearothermophilus nitric oxide synthase (gsNOS). The amplitudes of the two observed kinetics phases, which are insensitive to CO concentration, depend on enzyme concentration. We suggest that two forms of gsNOS are in equilibrium under the conditions employed (6.1-27 µM gsNOS with 20 or 100% CO atmosphere). The kinetics of CO rebinding to the heme do not depend on the identity of the NO-gate residues at positions 134 and 223.

Entities:  

Keywords:  Nitric oxide synthase; carbon monoxide; heme proteins

Year:  2013        PMID： 23976816      PMCID： PMC3747573          DOI： 10.1016/j.poly.2012.08.079

Source DB:  PubMed          Journal:  Polyhedron        ISSN： 0277-5387            Impact factor:   3.052


  37 in total

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