| Literature DB >> 23975811 |
Pauline Aubel1, Bertrand Pain.
Abstract
Embryonic stem (ES) cells are unique models for investigating early development and cell differentiation. First identified in mouse and later in other mammals, these cells have also been isolated in avian species. Here, using chicken as a model, we describe a set of protocols allowing the isolation, maintenance, genetic modification, differentiation, and injection of the chicken embryonic stem (cES) cells into embryos for obtaining chimeric animals.Entities:
Mesh:
Year: 2013 PMID: 23975811 DOI: 10.1007/978-1-62703-628-3_11
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745