| Literature DB >> 23973738 |
Marcos Pita1, Cristina Gutierrez-Sanchez, Miguel D Toscano, Sergey Shleev, Antonio L De Lacey.
Abstract
Gold disk electrodes modified with gold nanoparticles have been used as a scaffold for the covalent immobilization of bilirubin oxidase. The nanostructured bioelectrodes were tested as mediator-less biosensors for oxygen in a buffer that mimics the content and the composition of human physiological fluids. Chronoamperometry measurements showed a detection limit towards oxygen of 6 ± 1 μM with a linear range of 6-300 μM, i.e. exceeding usual physiological ranges of oxygen in human tissues and fluids. The biosensor presented is the first ever-reported oxygen amperometric biosensor based on direct electron transfer of bilirubin oxidase.Entities:
Keywords: Bilirubin oxidase; Biocatalysis; Biosensor; Direct electron transfer; Gold nanoparticles
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Year: 2013 PMID： 23973738 DOI： 10.1016/j.bioelechem.2013.07.001
Source DB: PubMed Journal: Bioelectrochemistry ISSN： 1567-5394 Impact factor: 5.373