Literature DB >> 239708

The formation of mono-N-acetylhexosamine derivatives of dolichol diphosphate by pig liver microsomal fractions.

G Palamarczyk, F W Hemming.   

Abstract

Incubation of pig liver microsomal preparations with UDP-N[U-14C]acetylglucosamine yields a 14C-labelled lipid. The requirement for Mn2+, the pH optimum, time-dependence and the reversibility by UMP of the transferase are reported. Evidence is presented in favour of the lipid being a mixture of dolichol diphosphate N-[14C]acetylglucosamine and dolichol diphosphate N-[14C]acetylmannosamine. Available data suggest that the epimerization takes place while the hexosamine is bound in this lipid-soluble form. The N-acetylmannosamine appeared not be be released into the medium. The subfractionation of the microsomal fraction to separate transferase activity from membrane-bound beta-N-acetylglucosaminidase activity is also reported.

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Year:  1975        PMID： 239708      PMCID： PMC1165532          DOI： 10.1042/bj1480245

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  14 in total

1.  A simple method for the isolation and purification of total lipides from animal tissues.

Authors:  J FOLCH; M LEES; G H SLOANE STANLEY
Journal:  J Biol Chem       Date:  1957-05       Impact factor: 5.157

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Biosynthesis of a P1-2-acetamido-2-deoxy-D-glucosyl P2-polyisoprenyl pyrophosphate by calf pancreas microsomes.

Authors:  M A Ghalambor; C D Warren; R W Jeanloz
Journal:  Biochem Biophys Res Commun       Date:  1974-01-23       Impact factor: 3.575

4.  The unambiguous characterization of dolichol phosphate mannose as a product of mannosyl transferase in pig liver endoplasmic reticulum.

Authors:  P J Evans; F W Hemming
Journal:  FEBS Lett       Date:  1973-05-01       Impact factor: 4.124

5.  Incorporation of carbohydrates into endogenous acceptors of liver microsomal fractions.

Authors:  M Tetas; H Chao; J Molnar
Journal:  Arch Biochem Biophys       Date:  1970-05       Impact factor: 4.013

6.  Mannose transfer to lipid linked di-N-acetylchitobiose.

Authors:  J A Levy; H Carminatti; A I Cantarella; N H Behrens; L F Leloir; E Tábora
Journal:  Biochem Biophys Res Commun       Date:  1974-09-09       Impact factor: 3.575

7.  The biosynthesis of a N,N'-diacetylchitobiose containing lipid by liver microsomes. A probable dolichol pyrophosphate derivative.

Authors:  L F Leloir; R J Staneloni; H Carminatti; N H Behrens
Journal:  Biochem Biophys Res Commun       Date:  1973-06-19       Impact factor: 3.575

8.  The role of dolichol monophosphate in sugar transfer.

Authors:  N H Behrens; A J Parodi; L F Leloir; C R Krisman
Journal:  Arch Biochem Biophys       Date:  1971-04       Impact factor: 4.013

9.  The transfer of mannose from guanosine diphosphate mannose to dolichol phosphate and protein by pig liver endoplasmic reticulum.

Authors:  J B Richards; F W Hemming
Journal:  Biochem J       Date:  1972-11       Impact factor: 3.857

10.  Golgi fractions prepared from rat liver homogenates. I. Isolation procedure and morphological characterization.

Authors:  J H Ehrenreich; J J Bergeron; P Siekevitz; G E Palade
Journal:  J Cell Biol       Date:  1973-10       Impact factor: 10.539
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  5 in total

1.  Glycoprotein Biosynthesis in Cotyledons of Pisum sativum L: Involvement of Lipid-linked Intermediates.

Authors:  L Beevers; R M Mense
Journal:  Plant Physiol       Date:  1977-11       Impact factor: 8.340

2.  The effects of triton X-100 on the transfer of mannose, glucose and n-acetylglusomine phosphate to dolichol monophosphate by preparations of rough and smooth endoplasmic reticulum and of mitochondria of rat liver.

Authors:  T Coolbear; S Mookerjea; F W Hemming
Journal:  Biochem J       Date:  1979-11-15       Impact factor: 3.857

3.  Incorporation of N-acetylglucosamine into endogenous acceptors of rough microsomes from rat liver: stimulation by GTP after treatment with pyrophosphate.

Authors:  D Godelaine; H Beaufay; M Wibo
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

4.  Polyisoprenoid glycolipids involved in glycoprotein biosynthesis.

Authors:  J J Lucas; C J Waechter
Journal:  Mol Cell Biochem       Date:  1976-04-28       Impact factor: 3.396

5.  Inhibition of Dephosphorylation of Dolichyl Diphosphate Alters the Synthesis of Dolichol and Hinders Protein N-Glycosylation and Morphological Transitions in Candida albicans.

Authors:  Anna Janik; Monika Niewiadomska; Urszula Perlińska-Lenart; Jacek Lenart; Damian Kołakowski; Karolina Skorupińska-Tudek; Ewa Swiezewska; Joanna S Kruszewska; Grażyna Palamarczyk
Journal:  Int J Mol Sci       Date:  2019-10-12       Impact factor: 5.923
  5 in total

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