Literature DB >> 239695

Purification and heterogeneity of inorganic pyrophosphatase of pig scapula cartilage.

R Felix, H Fleisch.   

Abstract

Inorganic pyrophosphatase (pyrophosphate phosphohydrolase; EC 3.6.1.1) was purified from pig scapula cartilage by fractionation with N-cetylpyridinium chloride and (NH4)2SO4, followed by ion-exchange and gel-filtration chromatography. Enzyme preparations of high purity were obtained, with specific activities (100-400 units/mg) higher than those previously described for mammalian pyrophosphatases. The enzyme activity could be separated into several subfractions on ion-exchange columns.

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Year:  1975        PMID： 239695      PMCID： PMC1165379          DOI： 10.1042/bj1470103

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

1.  THE SUBCELLULAR DISTRIBUTION OF INORGANIC PYROPHOSPHATASE IN PIGEON PANCREAS.

Authors:  G D PYNES; E S YOUNATHAN
Journal:  Biochim Biophys Acta       Date:  1964-10-23

2.  Sub-cellular distribution of ratliver inorganic pyrophosphatase activity.

Authors:  R C NORDLIE; H A LARDY
Journal:  Biochim Biophys Acta       Date:  1961-06-10

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Properties of inorganic pyrophosphatase of pig scapula cartilage.

Authors:  R Felix; H Fleisch
Journal:  Biochem J       Date:  1975-04       Impact factor: 3.857

5.  On the origin of "isozymes" of inorganic pyrophosphatase obtained from baker's yeast.

Authors:  R Eifler; V Hahn; I Hermann
Journal:  Acta Biol Med Ger       Date:  1972

6.  Determination of orthophosphate in the presence of inorganic pyrophosphate in the assay of inorganic pyrophosphatase activity.

Authors:  J Wöltgens; W Ahsmann
Journal:  Anal Biochem       Date:  1970-06       Impact factor: 3.365

7.  [Properties of a soluble inorganic pyrophosphatase from rat liver cell nuclei].

Authors:  K Kesselring; G Siebert
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1967-05

8.  Purification and some properties of inorganic pyrophosphatase from human erythrocytes.

Authors:  G D Pynes; E S Younathan
Journal:  J Biol Chem       Date:  1967-05-10       Impact factor: 5.157

9.  Crystalline inorganic pyrophosphatase isolated from baker's yeast.

Authors:  M KUNITZ
Journal:  J Gen Physiol       Date:  1952-01       Impact factor: 4.086

10.  Inorganic pyrophosphatase of rat liver mitochondria. Correlation of latency with catalytic properties and intramitochondrial location.

Authors:  L Schick; L G Butler
Journal:  J Cell Biol       Date:  1969-07       Impact factor: 10.539
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  7 in total

1.  Properties of inorganic pyrophosphatase of pig scapula cartilage.

Authors:  R Felix; H Fleisch
Journal:  Biochem J       Date:  1975-04       Impact factor: 3.857

2.  Proceedings of a symposium on crystal-related arthropathies. 22 October and 23 October, 1982, Bristol Polytechnic, Bristol.

Authors: 
Journal:  Ann Rheum Dis       Date:  1983-08       Impact factor: 19.103

Review 3.  Pathogenesis of chondrocalcinosis and pseudogout. Metabolism of inorganic pyrophosphate and production of calcium pyrophosphate dihydrate crystals.

Authors:  A Caswell; D F Guilland-Cumming; P R Hearn; M K McGuire; R G Russell
Journal:  Ann Rheum Dis       Date:  1983-08       Impact factor: 19.103

4.  Purification of alkaline phosphatase from extracellular vesicles of fracture callus cartilage.

Authors:  S E Kahn; A M Jafri; N J Lewis; C Arsenis
Journal:  Calcif Tissue Res       Date:  1978-02-28

5.  Activation and specificity of alkaline phosphatase of a mineralizing collagen-rich system.

Authors:  J Althoff; P Quint; H J Höhling
Journal:  Experientia       Date:  1978-06-15

6.  Matrix vesicles in chicken epiphyseal cartilage. Separation from lysosomes and the distribution of inorganic pyrophosphatase activity.

Authors:  H K Väänänen; L K Korhonen
Journal:  Calcif Tissue Int       Date:  1979-08-24       Impact factor: 4.333

7.  Separation of odontoblast Ca2+-ATPase and alkaline phosphatase.

Authors:  G Granström; M Jontell; A Linde
Journal:  Calcif Tissue Int       Date:  1979-07-03       Impact factor: 4.333
  7 in total

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