Literature DB >> 23966268

A novel method for sample delivery and testing of whole blood: gel strip PCR for point of care (POC) molecular diagnostics.

Dammika P Manage1, Jana Lauzon, Alexey Atrazhev, Xiaoli Pang, Linda M Pilarski.   

Abstract

Testing of whole blood in miniaturized PCR is compromised by the opaque nature of whole blood that leads to physical masking of a fluorescent signal. We demonstrate a method to perform real-time PCR with whole blood that avoids interference from the opacity of whole blood.

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Year:  2013        PMID： 23966268     DOI： 10.1039/c3lc50755f

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  7 in total

1.  Probe-free real-time reverse transcription polymerase chain reaction assays for the detection and typing of porcine reproductive and respiratory syndrome virus in Canada.

Authors:  Michael Eschbaumer; Wansi May Li; Kerstin Wernike; Frank Marshall; Markus Czub
Journal:  Can J Vet Res       Date:  2015-07       Impact factor: 1.310

2.  Detection of pathogenic Escherichia coli on potentially contaminated beef carcasses using cassette PCR and conventional PCR.

Authors:  Dammika P Manage; Jana Lauzon; Christina M Jones; Patrick J Ward; Linda M Pilarski; Patrick M Pilarski; Lynn M McMullen
Journal:  BMC Microbiol       Date:  2019-07-30       Impact factor: 3.605

Review 3.  Advances in Directly Amplifying Nucleic Acids from Complex Samples.

Authors:  Faye M Walker; Kuangwen Hsieh
Journal:  Biosensors (Basel)       Date:  2019-09-30

4.  Alberta Provincial Pediatric EnTeric Infection TEam (APPETITE): epidemiology, emerging organisms, and economics.

Authors:  Stephen B Freedman; Bonita E Lee; Marie Louie; Xiao-Li Pang; Samina Ali; Andy Chuck; Linda Chui; Gillian R Currie; James Dickinson; Steven J Drews; Mohamed Eltorki; Tim Graham; Xi Jiang; David W Johnson; James Kellner; Martin Lavoie; Judy MacDonald; Shannon MacDonald; Lawrence W Svenson; James Talbot; Phillip Tarr; Raymond Tellier; Otto G Vanderkooi
Journal:  BMC Pediatr       Date:  2015-07-31       Impact factor: 2.125

5.  A lab-on-chip for malaria diagnosis and surveillance.

Authors:  Brian J Taylor; Anita Howell; Kimberly A Martin; Dammika P Manage; Walter Gordy; Stephanie D Campbell; Samantha Lam; Albert Jin; Spencer D Polley; Roshini A Samuel; Alexey Atrazhev; Alex J Stickel; Josephine Birungi; Anthony K Mbonye; Linda M Pilarski; Jason P Acker; Stephanie K Yanow
Journal:  Malar J       Date:  2014-05-09       Impact factor: 2.979

6.  Transformation of personal computers and mobile phones into genetic diagnostic systems.

Authors:  Faye M Walker; Kareem M Ahmad; Michael Eisenstein; H Tom Soh
Journal:  Anal Chem       Date:  2014-08-31       Impact factor: 6.986

7.  Monitoring food pathogens: Novel instrumentation for cassette PCR testing.

Authors:  Darin Hunt; Curtis Figley; Dammika P Manage; Jana Lauzon; Rachel Figley; Linda M Pilarski; Lynn M McMullen; Patrick M Pilarski
Journal:  PLoS One       Date:  2018-05-10       Impact factor: 3.240
  7 in total

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