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Literature DB >> 23964359

Ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification.

Guohua Zhou¹, Xian Zhang, Xinghu Ji, Zhike He.

Abstract

Terminal protection of small molecule linked DNA assays amplified by Exo III-aided DNA recycling is developed for the detection of streptavidin-biotin interaction, with a detection limit of 0.8 fM streptavidin in the "turn-on" state, indicating that the proposed method is an ultrasensitive platform for the detection of small molecule-protein interactions.

Entities: Chemical

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Year: 2013 PMID： 23964359 DOI： 10.1039/c3cc43621g

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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Cited

5 in total

1. A cationic conjugated polymer coupled with exonuclease I: application to the fluorometric determination of protein and cell imaging.

Authors: Yufei Liu; Liyun Gao; Huijuan Yan; Jingfang Shangguan; Zhen Zhang; Xia Xiang
Journal: Mikrochim Acta Date: 2018-01-16 Impact factor: 5.833

2. Molecular machine and gold/graphene quantum dot hybrid based dual amplification strategy for voltammetric detection of VEGF165.

Authors: Chu Hongxia; Li Zaijun; Li Ruiyi; Wang Guangli; Gu Zhiguo
Journal: Mikrochim Acta Date: 2019-03-15 Impact factor: 5.833

Ultrasensitive detection of small molecule-protein interaction via terminal protection of small molecule linked DNA and Exo III-aided DNA recycling amplification.

1. A cationic conjugated polymer coupled with exonuclease I: application to the fluorometric determination of protein and cell imaging.

2. Molecular machine and gold/graphene quantum dot hybrid based dual amplification strategy for voltammetric detection of VEGF165.

Review 3. Terminal protection of small molecule-linked DNA for small molecule-protein interaction assays.

4. Protein-induced fluorescence enhancement for a simple and universal detection of protein/small molecule interactions.

5. Label-Free Fluorescence Molecular Beacon Probes Based on G-Triplex DNA and Thioflavin T for Protein Detection.