Literature DB >> 23964356

RT-isoPCR: nested, high multiplex mRNA amplification.

Martin Jensen Søe1, Peter Warthoe.   

Abstract

RT-isoPCR provides multiplex amplification of mRNA targets using a first-stage multiplex RT-PCR reaction with subsequent isothermal amplification for individual target loci detection. We demonstrate detection of 24 mRNA targets with high specificity and sensitivity without compromising sample variation or introducing biases between targets.

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Year:  2013        PMID: 23964356     DOI: 10.1039/c3an00803g

Source DB:  PubMed          Journal:  Analyst        ISSN: 0003-2654            Impact factor:   4.616


  1 in total

1.  Two-Stage Isothermal Enzymatic Amplification for Concurrent Multiplex Molecular Detection.

Authors:  Jinzhao Song; Changchun Liu; Michael G Mauk; Shelley C Rankin; James B Lok; Robert M Greenberg; Haim H Bau
Journal:  Clin Chem       Date:  2017-01-10       Impact factor: 8.327

  1 in total

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