OBJECTIVE: PGC-1α is a transcriptional co-activator and master regulator of mitochondrial biogenesis. While extensively studied in skeletal and cardiac muscle, recent findings suggest that white adipose tissue PGC-1α plays an important role in regulating glucose homeostasis. The purpose of the present investigation was to evaluate the role of AMPK in regulating PGC-1α and mitochondrial enzymes in mouse epididymal and inguinal subcutaneous adipose tissue. METHODS: Mitochondrial protein content and norepinephrine and CL 316,243-induced PGC-1α mRNA expression were studied in mouse epididymal and inguinal adipose tissue from wild-type and AMPK β1(-/-) mice. RESULTS: The protein content and phosphorylation of AMPKα was reduced in epididymal adipose tissue from AMPK β1(-/-) compared to WT mice, concomitant with decreases in PGC-1α and mitochondrial marker proteins. Norepinephrine and CL 316,243-mediated induction of PGC-1α were decreased in cultured epididymal adipose tissue from AMPK β1(-/-) relative to WT mice. In inguinal adipose tissue from AMPK β1(-/-) mice, mitochondrial marker protein content and norepinephrine and CL 316,243-mediated increases in PGC-1α were normal despite reductions in the content and phosphorylation of AMPKα. CONCLUSIONS: Norepinephrine- and CL 316,243-mediated induction of PGC-1α and mitochondrial protein expression is regulated by AMPK in epididymal, but not inguinal adipose tissue.
OBJECTIVE: PGC-1α is a transcriptional co-activator and master regulator of mitochondrial biogenesis. While extensively studied in skeletal and cardiac muscle, recent findings suggest that white adipose tissue PGC-1α plays an important role in regulating glucose homeostasis. The purpose of the present investigation was to evaluate the role of AMPK in regulating PGC-1α and mitochondrial enzymes in mouseepididymal and inguinal subcutaneous adipose tissue. METHODS: Mitochondrial protein content and norepinephrine and CL 316,243-induced PGC-1α mRNA expression were studied in mouseepididymal and inguinal adipose tissue from wild-type and AMPK β1(-/-) mice. RESULTS: The protein content and phosphorylation of AMPKα was reduced in epididymal adipose tissue from AMPK β1(-/-) compared to WT mice, concomitant with decreases in PGC-1α and mitochondrial marker proteins. Norepinephrine and CL 316,243-mediated induction of PGC-1α were decreased in cultured epididymal adipose tissue from AMPK β1(-/-) relative to WT mice. In inguinal adipose tissue from AMPK β1(-/-) mice, mitochondrial marker protein content and norepinephrine and CL 316,243-mediated increases in PGC-1α were normal despite reductions in the content and phosphorylation of AMPKα. CONCLUSIONS:Norepinephrine- and CL 316,243-mediated induction of PGC-1α and mitochondrial protein expression is regulated by AMPK in epididymal, but not inguinal adipose tissue.
Authors: Tara L MacDonald; Rebecca MacPherson; Laura Castellani; Daniel Cervone; Eoin Anderson; David C Wright; David J Dyck Journal: Adipocyte Date: 2017-01-27 Impact factor: 4.534
Authors: Emilio P Mottillo; Eric M Desjardins; Justin D Crane; Brennan K Smith; Alex E Green; Serge Ducommun; Tora I Henriksen; Irena A Rebalka; Aida Razi; Kei Sakamoto; Camilla Scheele; Bruce E Kemp; Thomas J Hawke; Joaquin Ortega; James G Granneman; Gregory R Steinberg Journal: Cell Metab Date: 2016-07-12 Impact factor: 27.287
Authors: Rebecca E K MacPherson; Steven M Dragos; Sofhia Ramos; Charles Sutton; Scott Frendo-Cumbo; Laura Castellani; Matthew J Watt; Christopher G R Perry; David M Mutch; David C Wright Journal: Am J Physiol Cell Physiol Date: 2016-06-29 Impact factor: 4.249
Authors: Laura Castellani; Jared Root-Mccaig; Scott Frendo-Cumbo; Marie-Soleil Beaudoin; David C Wright Journal: J Appl Physiol (1985) Date: 2014-03-27
Authors: Rebecca E K MacPherson; Laura Castellani; Marie-Soleil Beaudoin; David C Wright Journal: Am J Physiol Endocrinol Metab Date: 2014-08-05 Impact factor: 4.310