| Literature DB >> 23961718 |
Yubiao Guo1, Junna Hou, Yifeng Luo, Dujuan Wang.
Abstract
Entities:
Year: 2013 PMID: 23961718 PMCID: PMC3847589 DOI: 10.1186/1475-2867-13-84
Source DB: PubMed Journal: Cancer Cell Int ISSN: 1475-2867 Impact factor: 5.722
Figure 1siRNA-mediated knockdown of MIF expression in H460 cells detected by Western blot. (A) is a western blot of MIF expression in H460 cells treated with NC and two MIF siRNAs for 48 hours. In MIF siRNA-transfected H460 cells, we observed an approximately two (siRNA 1) to five (siRNA2) fold weaker signal of MIF protein expression compared with the negative control (NC) group normalized to the expression of β-actin. (B) is a densitometric analysis of the western blot. This shows the relative densities of protein levels which were measured by Quantity One (Bio-Rad company) software.