| Literature DB >> 23961239 |
Kandhasamy Sowndhararajan1, Sun Chul Kang.
Abstract
The present study was carried out to determine the free radical scavenging potential of culture filtrate of Streptomyces sp. AM-S1. Antioxidant activity of culture filtrate, lyophilized culture filtrate and ethyl acetate extract of Streptomyces sp. AM-S1 was determined by various in vitro assays such as ferric reducing power assay, phosphomolybdenum reduction, DPPH and ABTS radical scavenging activities. The results revealed that the culture filtrate of Streptomyces sp. AM-S1 effectively scavenged DPPH (IC50 90.2 μl/ml) and ABTS (IC50 13.2 μl/ml) radicals in a concentration dependent manner. In all the assays, ethyl acetate extract registered higher antioxidant activity when compared with the lyophilized culture filtrate (LCF). In addition, ethyl acetate extract (1123.4 μmole Fe(II)/mg extract) exhibited higher ferric reducing activity than the standard BHA (814.4 μmole Fe(II)/mg extract). Further works are needed on the isolation and identification of antioxidant molecules from the ethyl acetate extract of Streptomyces sp. AM-S1 culture filtrate.Entities:
Keywords: ABTS; Antioxidant; DPPH; Ferric reducing; Streptomyces sp. AM-S1
Year: 2013 PMID： 23961239 PMCID： PMC3730576 DOI： 10.1016/j.sjbs.2012.12.003
Source DB: PubMed Journal: Saudi J Biol Sci ISSN： 1319-562X Impact factor: 4.219