| Literature DB >> 23960722 |
Imran Ali1, Hassan Y Aboul-Enein, Prashant Singh, Rakesh Singh, Bhavtosh Sharma.
Abstract
After the success of human genome project, proteome is a new emerging field of biochemistry as it provides the knowledge of enzymes (proteins) interactions with different body organs and medicines administrated into human body. Therefore, the study of proteomics is very important for the development of new and effective drugs to control many lethal diseases. In proteomics study, analyses of proteome is essential and significant from the pathological point of views, i.e., in several serious diseases such as cancer, Alzheimer's disease and aging, heart diseases and also for plant biology. The separation and identification of proteomics is a challenging job due to their complex structures and closely related physico-chemical behaviors. However, the recent advances in liquid chromatography make this job easy. Various kinds of liquid chromatography, along with different detectors and optimization strategies, have been discussed in this article. Besides, attempts have been made to include chirality concept in proteomics for understanding mechanism and medication of various disease controlled by different body proteins.Entities:
Keywords: 2D-nano LC, two-dimensional nano liquid chromatography quadrupole; ACN, acetonitrile; AIEC, anion exchange chromatography; CEC, capillary electro-chromatography; CIEF, capillary isoelectric focusing; CSF, cerebrospinal fluid; Chirality; EC, electro-chromatography; ESI-LC–MS, electrospray ionization liquid chromatography–mass spectrometry; FA, formic acid; FLP, FMRF amide-like peptide; FT-ICR-MS, ion cyclotron resonance-mass spectrometry; GPI-APs, glycosylphosphadylinositol anchored proteins; GSH, glutathione stimulating hormone; GSTs, glutathione-S-transferase isoenzyme; Gene; HFBA, heptafluorobutyric acid; HPLC, high performance liquid chromatography; ICAT, isotope coded affinity tag; IEF-SEC, isoelectrofocussing size-exclusion chromatography; IMCD, inner medullary collecting duct; LC-Q-TOF, liquid chromatography-quadrupole time-of-flight tandem mass; LC-dual ESI, liquid chromatography dual electrospray ionization-Fourier transform; LC–MS, liquid chromatography–mass spectrometry; Liquid chromatography; MALDI-TOF, matrix-assisted laser desorption/ionization-time-of flight; MFGM, milk fat globule membranes; MMA, mass measurement accuracy; MPC, mesenchymal progenitor cell; MS/MS, spectrometry; NLFs, Nasal lavage fluids; NLP, neuropeptide like protein; Nano detection; PC2, prohormone convertase-2; PS II, photosystem II; Preparation; Proteomics; Q-TOFMS/MS, time-of-flight tandem-mass spectrometry; RPLC, reversed phase liquid chromatography; SCX, strong cation exchange; SEC, size-exclusion chromatography; TFA, trifluoroacetic acid; TIC, total ion current; TRAF, tumor necrosis factor receptor
Year: 2010 PMID： 23960722 PMCID： PMC3730981 DOI： 10.1016/j.jsps.2010.02.001
Source DB: PubMed Journal: Saudi Pharm J ISSN： 1319-0164 Impact factor: 4.330