Literature DB >> 23959986

The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting.

Shen Y Heazlewood1, Brenda Williams, Melonie J Storan, Susan K Nilsson.   

Abstract

Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been difficult to isolate in large numbers. Here in, we report a fluorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).

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Year:  2013        PMID: 23959986     DOI: 10.1007/978-1-62703-508-8_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  6 in total

1.  Improved quantitative analysis of primary bone marrow megakaryocytes utilizing imaging flow cytometry.

Authors:  Lisa M Niswander; Kathleen E McGrath; John C Kennedy; James Palis
Journal:  Cytometry A       Date:  2014-01-16       Impact factor: 4.355

2.  TNF-α-driven inflammation and mitochondrial dysfunction define the platelet hyperreactivity of aging.

Authors:  Pavel Davizon-Castillo; Brandon McMahon; Sonia Aguila; David Bark; Katrina Ashworth; Ayed Allawzi; Robert A Campbell; Emilie Montenont; Travis Nemkov; Angelo D'Alessandro; Nathan Clendenen; Lauren Shih; Natalie A Sanders; Kelly Higa; Allaura Cox; Zavelia Padilla-Romo; Giovanni Hernandez; Eric Wartchow; George D Trahan; Eva Nozik-Grayck; Kenneth Jones; Eric M Pietras; James DeGregori; Matthew T Rondina; Jorge Di Paola
Journal:  Blood       Date:  2019-07-16       Impact factor: 22.113

Review 3.  Platelet Heterogeneity in Myeloproliferative Neoplasms.

Authors:  Sally Thomas; Anandi Krishnan
Journal:  Arterioscler Thromb Vasc Biol       Date:  2021-10-07       Impact factor: 10.514

4.  Loss of Tet2 affects platelet function but not coagulation in mice.

Authors:  Bichen Wang; Meijuan Xia; Ting Chen; Mengke Li; Deyang Shi; Xiaomin Wang; Aiming Pang; Jiaxi Zhou; Weiping Yuan; Yajing Chu
Journal:  Blood Sci       Date:  2020-10-23

Review 5.  The Analysis of the Human Megakaryocyte and Platelet Coding Transcriptome in Healthy and Diseased Subjects.

Authors:  Koenraad De Wispelaere; Kathleen Freson
Journal:  Int J Mol Sci       Date:  2022-07-11       Impact factor: 6.208

Review 6.  Megakaryocyte and Platelet Transcriptomics for Discoveries in Human Health and Disease.

Authors:  Pavel Davizon-Castillo; Jesse W Rowley; Matthew T Rondina
Journal:  Arterioscler Thromb Vasc Biol       Date:  2020-04-16       Impact factor: 10.514

  6 in total

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