Literature DB >> 23959979

Visualization of adult stem cells within their niches using the Drosophila germline as a model system.

Annekatrin König1, Halyna R Shcherbata.   

Abstract

The germaria of the fruit fly Drosophila melanogaster present an excellent model to study germline stem cell-niche interactions. Two to three adult stem cells are surrounded by a number of somatic cells that form the niche. Here we describe how Drosophilae germaria can be dissected and specifically immuno-stained to allow for identification and analysis of both the adult stem cells and their somatic niche cells.

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Year:  2013        PMID: 23959979     DOI: 10.1007/978-1-62703-508-8_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  5 in total

1.  RNAi-Based Techniques for the Analysis of Gene Function in Drosophila Germline Stem Cells.

Authors:  Amelia J Blake; Danielle S Finger; Victoria L Hardy; Elizabeth T Ables
Journal:  Methods Mol Biol       Date:  2017

2.  Soma influences GSC progeny differentiation via the cell adhesion-mediated steroid-let-7-Wingless signaling cascade that regulates chromatin dynamics.

Authors:  Annekatrin König; Halyna R Shcherbata
Journal:  Biol Open       Date:  2015-02-06       Impact factor: 2.422

3.  Stereotypical architecture of the stem cell niche is spatiotemporally established by miR-125-dependent coordination of Notch and steroid signaling.

Authors:  Andriy S Yatsenko; Halyna R Shcherbata
Journal:  Development       Date:  2018-02-08       Impact factor: 6.868

4.  Ultrastructural analysis of different human mesenchymal stem cells after in vitro expansion: a technical review.

Authors:  M Miko; L Danišovič; A Majidi; I Varga
Journal:  Eur J Histochem       Date:  2015-10-26       Impact factor: 3.188

5.  A genome-wide survey of sexually dimorphic expression of Drosophila miRNAs identifies the steroid hormone-induced miRNA let-7 as a regulator of sexual identity.

Authors:  Delphine Fagegaltier; Annekatrin König; Assaf Gordon; Eric C Lai; Thomas R Gingeras; Gregory J Hannon; Halyna R Shcherbata
Journal:  Genetics       Date:  2014-07-31       Impact factor: 4.562

  5 in total

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