Literature DB >> 23955743

Generation and analysis of biosensors to measure mechanical forces within cells.

Katharina Austen1, Carleen Kluger, Andrea Freikamp, Anna Chrostek-Grashoff, Carsten Grashoff.   

Abstract

The inability to measure mechanical forces within cells has been limiting our understanding of how mechanical information is processed on the molecular level. In this chapter, we describe a method that allows the analysis of force propagation across distinct proteins within living cells using Förster resonance energy transfer (FRET)-based biosensors.

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Year:  2013        PMID: 23955743     DOI: 10.1007/978-1-62703-604-7_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  13 in total

1.  Construction, imaging, and analysis of FRET-based tension sensors in living cells.

Authors:  Andrew S LaCroix; Katheryn E Rothenberg; Matthew E Berginski; Aarti N Urs; Brenton D Hoffman
Journal:  Methods Cell Biol       Date:  2015-01-08       Impact factor: 1.441

2.  Tunable molecular tension sensors reveal extension-based control of vinculin loading.

Authors:  Andrew S LaCroix; Andrew D Lynch; Matthew E Berginski; Brenton D Hoffman
Journal:  Elife       Date:  2018-07-19       Impact factor: 8.140

3.  Extending the Capabilities of Molecular Force Sensors via DNA Nanotechnology.

Authors:  Susana M Beltrán; Marvin J Slepian; Rebecca E Taylor
Journal:  Crit Rev Biomed Eng       Date:  2020

4.  Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1.

Authors:  Pia Ringer; Andreas Weißl; Anna-Lena Cost; Andrea Freikamp; Benedikt Sabass; Alexander Mehlich; Marc Tramier; Matthias Rief; Carsten Grashoff
Journal:  Nat Methods       Date:  2017-09-18       Impact factor: 28.547

5.  Rap1 and membrane lipids cooperatively recruit talin to trigger integrin activation.

Authors:  Thomas Bromberger; Liang Zhu; Sarah Klapproth; Jun Qin; Markus Moser
Journal:  J Cell Sci       Date:  2019-11-01       Impact factor: 5.285

6.  Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads.

Authors:  Angélique Millon-Frémillon; Julien Aureille; Christophe Guilluy
Journal:  J Vis Exp       Date:  2017-03-08       Impact factor: 1.355

7.  Structure of Rap1b bound to talin reveals a pathway for triggering integrin activation.

Authors:  Liang Zhu; Jun Yang; Thomas Bromberger; Ashley Holly; Fan Lu; Huan Liu; Kevin Sun; Sarah Klapproth; Jamila Hirbawi; Tatiana V Byzova; Edward F Plow; Markus Moser; Jun Qin
Journal:  Nat Commun       Date:  2017-11-23       Impact factor: 14.919

8.  Mechanical loading of desmosomes depends on the magnitude and orientation of external stress.

Authors:  Andrew J Price; Anna-Lena Cost; Hanna Ungewiß; Jens Waschke; Alexander R Dunn; Carsten Grashoff
Journal:  Nat Commun       Date:  2018-12-11       Impact factor: 14.919

9.  Extracellular rigidity sensing by talin isoform-specific mechanical linkages.

Authors:  Katharina Austen; Pia Ringer; Alexander Mehlich; Anna Chrostek-Grashoff; Carleen Kluger; Christoph Klingner; Benedikt Sabass; Roy Zent; Matthias Rief; Carsten Grashoff
Journal:  Nat Cell Biol       Date:  2015-11-02       Impact factor: 28.824

Review 10.  Under Pressure: Mechanical Stress Management in the Nucleus.

Authors:  Néjma Belaadi; Julien Aureille; Christophe Guilluy
Journal:  Cells       Date:  2016-06-14       Impact factor: 6.600

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