J Wang1, Y Li, J Li. 1. Department of Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
Abstract
BACKGROUND: We sought to assess the expression of five cell stress molecules: Heat shock protein (HSP)-60, HSP-70, interferon (IFN)-α, rat retinoic acid early induible-1 like transcript (RRLT), and vasoactive intestinal peptide (VIP) during rat chronic small bowel allograft rejection. METHODS: A rat model of small bowel allograft rejection was induced on postoperative day 90 using F344 rat as donor and Lewis rat as recipient. RESULTS: We demonstrated increased expression of lamina propria HSP-60 (mean rank grade 8.3 and 3.2, respectively), graft RRLT (relative mRNA amount 5.1 ± 1.8 and 3.0 ± 1.3, respectively) and IFN-α (relative mRNA amount 108.3 ± 49.2 and 59.2 ± 22.0, respectively) in allograft group compared with the isograft group. CONCLUSION: These data suggest the presence of cell stress during chronic small bowel allograft rejection.
BACKGROUND: We sought to assess the expression of five cell stress molecules: Heat shock protein (HSP)-60, HSP-70, interferon (IFN)-α, ratretinoic acid early induible-1 like transcript (RRLT), and vasoactive intestinal peptide (VIP) during rat chronic small bowel allograft rejection. METHODS: A rat model of small bowel allograft rejection was induced on postoperative day 90 using F344 rat as donor and Lewis rat as recipient. RESULTS: We demonstrated increased expression of lamina propria HSP-60 (mean rank grade 8.3 and 3.2, respectively), graft RRLT (relative mRNA amount 5.1 ± 1.8 and 3.0 ± 1.3, respectively) and IFN-α (relative mRNA amount 108.3 ± 49.2 and 59.2 ± 22.0, respectively) in allograft group compared with the isograft group. CONCLUSION: These data suggest the presence of cell stress during chronic small bowel allograft rejection.