OBJECTIVE: To assess follicular development after long-term xenotransplantation and exogenous stimulation of cryopreserved prepubertal ovarian tissue. DESIGN: Pilot study. SETTING: Gynecology research unit in a university hospital. PATIENT(S): Cryopreserved ovarian fragments were obtained from five prepubertal patients aged 7.2-12.2 years. INTERVENTION(S): Xenografting of frozen-thawed prepubertal ovarian fragments to SCID mice for 5 months and exogenous stimulation. MAIN OUTCOME MEASURE(S): Follicular density, morphology, proliferation, development. RESULT(S): Follicular density varied between 1.05 and 47.89 follicles/mm(2) in frozen-thawed ovarian tissue and 0.48 and 32.74 follicles/mm(2) in grafted prepubertal ovarian tissue. Growing follicles at the last stage of follicular development were observed at significantly higher proportions after grafting. No statistical difference was evidenced in the number of primordial follicles, representing the largest proportion of the follicle pool (99.51% before grafting and 92.46% after grafting). Ki67 and antimüllerian hormone expression were observed in these growing follicles. CONCLUSION(S): This is the first description of transplantation of human cryopreserved prepubertal ovarian tissue to mice, demonstrating that a very high number of follicles survive after transplantation and a large pool of primordial follicles remains dormant. Growing follicles were observed, proving the responsiveness of prepubertal ovarian tissue to gonadotropins.
OBJECTIVE: To assess follicular development after long-term xenotransplantation and exogenous stimulation of cryopreserved prepubertal ovarian tissue. DESIGN: Pilot study. SETTING: Gynecology research unit in a university hospital. PATIENT(S): Cryopreserved ovarian fragments were obtained from five prepubertal patients aged 7.2-12.2 years. INTERVENTION(S): Xenografting of frozen-thawed prepubertal ovarian fragments to SCIDmice for 5 months and exogenous stimulation. MAIN OUTCOME MEASURE(S): Follicular density, morphology, proliferation, development. RESULT(S): Follicular density varied between 1.05 and 47.89 follicles/mm(2) in frozen-thawed ovarian tissue and 0.48 and 32.74 follicles/mm(2) in grafted prepubertal ovarian tissue. Growing follicles at the last stage of follicular development were observed at significantly higher proportions after grafting. No statistical difference was evidenced in the number of primordial follicles, representing the largest proportion of the follicle pool (99.51% before grafting and 92.46% after grafting). Ki67 and antimüllerian hormone expression were observed in these growing follicles. CONCLUSION(S): This is the first description of transplantation of human cryopreserved prepubertal ovarian tissue to mice, demonstrating that a very high number of follicles survive after transplantation and a large pool of primordial follicles remains dormant. Growing follicles were observed, proving the responsiveness of prepubertal ovarian tissue to gonadotropins.
Authors: A Balduzzi; J-H Dalle; K Jahnukainen; M von Wolff; G Lucchini; M Ifversen; K T Macklon; C Poirot; T Diesch; A Jarisch; D Bresters; I Yaniv; B Gibson; A M Willasch; R Fadini; L Ferrari; A Lawitschka; A Ahler; N Sänger; S Corbacioglu; M Ansari; R Moffat; A Dalissier; E Beohou; P Sedlacek; A Lankester; C D De Heredia Rubio; K Vettenranta; J Wachowiak; A Yesilipek; E Trigoso; T Klingebiel; C Peters; P Bader Journal: Bone Marrow Transplant Date: 2017-07-24 Impact factor: 5.483
Authors: Monica M Laronda; Francesca E Duncan; Jessica E Hornick; Min Xu; Jennifer E Pahnke; Kelly A Whelan; Lonnie D Shea; Teresa K Woodruff Journal: J Assist Reprod Genet Date: 2014-05-21 Impact factor: 3.412
Authors: L Bastings; J Liebenthron; J R Westphal; C C M Beerendonk; H van der Ven; B Meinecke; M Montag; D D M Braat; R Peek Journal: J Assist Reprod Genet Date: 2014-06-14 Impact factor: 3.412
Authors: J-H Dalle; G Lucchini; A Balduzzi; M Ifversen; K Jahnukainen; K T Macklon; A Ahler; A Jarisch; M Ansari; E Beohou; D Bresters; S Corbacioglu; A Dalissier; C Diaz de Heredia Rubio; T Diesch; B Gibson; T Klingebiel; A Lankester; A Lawitschka; R Moffat; C Peters; C Poirot; N Saenger; P Sedlacek; E Trigoso; K Vettenranta; J Wachowiak; A Willasch; M von Wolff; I Yaniv; A Yesilipek; P Bader Journal: Bone Marrow Transplant Date: 2017-03-13 Impact factor: 5.483
Authors: Francesca E Duncan; Mary Ellen Pavone; Alexander H Gunn; Sherif Badawy; Clarisa Gracia; Jill P Ginsberg; Barbara Lockart; Yasmin Gosiengfiao; Teresa K Woodruff Journal: J Adolesc Young Adult Oncol Date: 2015-12 Impact factor: 2.223