BACKGROUND: Calcium and its signaling have a role in adipogenesis. Transient Receptor Potential (TRP) channels are non-selective cation channels with a high permeability to calcium. METHODS: In the present study the expression of multiple TRP channels on mouse 3T3-L1 preadipocyte and adipocyte cells, white (WAT) and brown (BAT) adipose tissues was investigated using real time PCR (RT-PCR). RESULTS: TRPV1, TRPV3, TRPM8, TRPC4, TRPC6 were differentially expressed in preadipocytes and adipocytes suggesting their significance in adipogenesis. Genes for multiple TRP channels were also expressed in murine WAT and BAT, out of which TRPV4, TRPV6 and TRPC6 showed differential expression. CONCLUSION: Present study demonstrates the expression of TRP channels in mouse cell lines and adipose tissues.
BACKGROUND:Calcium and its signaling have a role in adipogenesis. Transient Receptor Potential (TRP) channels are non-selective cation channels with a high permeability to calcium. METHODS: In the present study the expression of multiple TRP channels on mouse 3T3-L1 preadipocyte and adipocyte cells, white (WAT) and brown (BAT) adipose tissues was investigated using real time PCR (RT-PCR). RESULTS:TRPV1, TRPV3, TRPM8, TRPC4, TRPC6 were differentially expressed in preadipocytes and adipocytes suggesting their significance in adipogenesis. Genes for multiple TRP channels were also expressed in murine WAT and BAT, out of which TRPV4, TRPV6 and TRPC6 showed differential expression. CONCLUSION: Present study demonstrates the expression of TRP channels in mouse cell lines and adipose tissues.
Authors: Katarina Ondrusova; Mohammad Fatehi; Amy Barr; Zofia Czarnecka; Wentong Long; Kunimasa Suzuki; Scott Campbell; Koenraad Philippaert; Matthew Hubert; Edward Tredget; Peter Kwan; Nicolas Touret; Martin Wabitsch; Kevin Y Lee; Peter E Light Journal: Sci Rep Date: 2017-11-27 Impact factor: 4.379