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Literature DB >> 23949737

RNA-binding protein RBM8A (Y14) and MAGOH localize to centrosome in human A549 cells.

Yasuhito Ishigaki¹, Yuka Nakamura, Takanori Tatsuno, Mitsumasa Hashimoto, Kuniyoshi Iwabuchi, Naohisa Tomosugi.

Abstract

RBM8A (Y14) is carrying RNA-binding motif and forms the tight heterodimer with MAGOH. The heterodimer is known to be a member of exon junction complex on exporting mRNA and is required for mRNA metabolisms such as splicing, mRNA export and nonsense-mediated mRNA decay. Almost all RBM8A-MAGOH complexes localize in nucleoplasm and shuttle between nuclei and cytoplasm for RNA metabolism. Recently, the abnormality of G2/M transition and aberrant centrosome regulation in RBM8A- or MAGOH-deficient cells has been reported. These results prompt us to the reevaluation of the localization of RBM8A-MAGOH in human cells. Interestingly, our immunostaining experiments showed the localization of these proteins in centrosome in addition to nuclei. Furthermore, the transiently expressed eYFP-tagged RBM8A and Flag-tagged MAGOH also co-localized with centrosome signals. In addition, the proximity ligation in situ assay was performed to detect the complex formation in centrosome. Our experiments clearly showed that Myc-tagged RBM8A and Flag-tagged MAGOH formed a complex in centrosome. GFP-tagged PLK1 also co-localized with Myc-RBM8A. Our results show that RBM8A-MAGOH complex is required for M-phase progression via direct localization to centrosome rather than indirect effect.

Entities: Gene Species

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Year: 2013 PMID： 23949737 DOI： 10.1007/s00418-013-1135-4

Source DB: PubMed Journal: Histochem Cell Biol ISSN： 0948-6143 Impact factor: 4.304

48 in total

1. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.

Authors: Michael D Blower; Maxence Nachury; Rebecca Heald; Karsten Weis
Journal: Cell Date: 2005-04-22 Impact factor: 41.582

2. Disassembly of exon junction complexes by PYM.

Authors: Niels H Gehring; Styliani Lamprinaki; Andreas E Kulozik; Matthias W Hentze
Journal: Cell Date: 2009-05-01 Impact factor: 41.582

3. A proximity ligation assay using transiently transfected, epitope-tagged proteins: application for in situ detection of dimerized receptor tyrosine kinases.

Authors: Aaron Gajadhar; Abhijit Guha
Journal: Biotechniques Date: 2010-02 Impact factor: 1.993

Review 4. EJCs at the heart of translational control.

Authors: Hervé Le Hir; Bertrand Séraphin
Journal: Cell Date: 2008-04-18 Impact factor: 41.582

5. Scanning electron microscopy with an ionic liquid reveals the loss of mitotic protrusions of cells during the epithelial-mesenchymal transition.

Authors: Yasuhito Ishigaki; Yuka Nakamura; Teruaki Takehara; Takeo Shimasaki; Takanori Tatsuno; Fumihide Takano; Yoshimichi Ueda; Yoshiharu Motoo; Tsutomu Takegami; Hideaki Nakagawa; Susumu Kuwabata; Noriko Nemoto; Naohisa Tomosugi; Shichiro Miyazawa
Journal: Microsc Res Tech Date: 2011-03-16 Impact factor: 2.769

6. The cryo-EM structure of the UPF-EJC complex shows UPF1 poised toward the RNA 3' end.

Authors: Roberto Melero; Gretel Buchwald; Raquel Castaño; Monika Raabe; David Gil; Melisa Lázaro; Henning Urlaub; Elena Conti; Oscar Llorca
Journal: Nat Struct Mol Biol Date: 2012-04-22 Impact factor: 15.369

7. Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex.

Authors: N Kataoka; M D Diem; V N Kim; J Yong; G Dreyfuss
Journal: EMBO J Date: 2001-11-15 Impact factor: 11.598

8. Direct observation of individual endogenous protein complexes in situ by proximity ligation.

Authors: Ola Söderberg; Mats Gullberg; Malin Jarvius; Karin Ridderstråle; Karl-Johan Leuchowius; Jonas Jarvius; Kenneth Wester; Per Hydbring; Fuad Bahram; Lars-Gunnar Larsson; Ulf Landegren
Journal: Nat Methods Date: 2006-10-29 Impact factor: 28.547

9. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome.

Authors: Y Zheng; M K Jung; B R Oakley
Journal: Cell Date: 1991-05-31 Impact factor: 41.582

10. Compound inheritance of a low-frequency regulatory SNP and a rare null mutation in exon-junction complex subunit RBM8A causes TAR syndrome.

Authors: Cornelis A Albers; Dirk S Paul; Harald Schulze; Kathleen Freson; Jonathan C Stephens; Peter A Smethurst; Jennifer D Jolley; Ana Cvejic; Myrto Kostadima; Paul Bertone; Martijn H Breuning; Najet Debili; Panos Deloukas; Rémi Favier; Janine Fiedler; Catherine M Hobbs; Ni Huang; Matthew E Hurles; Graham Kiddle; Ingrid Krapels; Paquita Nurden; Claudia A L Ruivenkamp; Jennifer G Sambrook; Kenneth Smith; Derek L Stemple; Gabriele Strauss; Chantal Thys; Chris van Geet; Ruth Newbury-Ecob; Willem H Ouwehand; Cedric Ghevaert
Journal: Nat Genet Date: 2012-02-26 Impact factor: 38.330

13 in total

RNA-binding protein RBM8A (Y14) and MAGOH localize to centrosome in human A549 cells.

1. A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.

2. Disassembly of exon junction complexes by PYM.

3. A proximity ligation assay using transiently transfected, epitope-tagged proteins: application for in situ detection of dimerized receptor tyrosine kinases.

Review 4. EJCs at the heart of translational control.

5. Scanning electron microscopy with an ionic liquid reveals the loss of mitotic protrusions of cells during the epithelial-mesenchymal transition.

6. The cryo-EM structure of the UPF-EJC complex shows UPF1 poised toward the RNA 3' end.

7. Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex.

8. Direct observation of individual endogenous protein complexes in situ by proximity ligation.

9. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome.

10. Compound inheritance of a low-frequency regulatory SNP and a rare null mutation in exon-junction complex subunit RBM8A causes TAR syndrome.

Review 1. The Histochemistry and Cell Biology pandect: the year 2014 in review.

2. Phosphorylation status of human RNA-binding protein 8A in cells and its inhibitory regulation by Magoh.

3. Prolonged Mitosis of Neural Progenitors Alters Cell Fate in the Developing Brain.

Review 4. Function and pathological implications of exon junction complex factor Y14.

5. Immunostaining and time-lapse analysis of vinblastine-induced paracrystal formation in human A549 cells.

6. Y14 governs p53 expression and modulates DNA damage sensitivity.

7. A network-based approach to dissect the cilia/centrosome complex interactome.

8. Nonsense-mediated mRNA decay factor Upf2 exists in both the nucleoplasm and the cytoplasm.

9. ATX-2, the C. elegans Ortholog of Human Ataxin-2, Regulates Centrosome Size and Microtubule Dynamics.

10. C-terminal short arginine/serine repeat sequence-dependent regulation of Y14 (RBM8A) localization.