PURPOSE: Intra-abdominal hypertension (IAH) causes lung dysfunction in patients after hemorrhagic shock resuscitation. We performed intra-abdominal volume increment (IAVI) to IAH and studied its effect on pulmonary function. METHODS:Eight minipigs established for IAH model by exsanquination until shock, incomplete blockage of portal vein and overload resuscitation were randomly divided into IAVI group (n=4) and sham-operated control group (n=4). Bladder pressure, arterial blood gas analysis and thoraco-abdominal computed tomography (CT) scans were measured. The minipigs were sacrificed 26 h after surgery, and lung samples were harvested for measuring the wet-to-dry weight ratio and hematoxylin-eosin staining. RESULTS: Compared with sham-operated control group, the respiratory rate and paCO2 remarkably decreased and paO2 notably increased at 8 and 12 h in the IAVI group. The bladder pressure also notably decreased at 8, 12, and 22 h after IAVI treatment. However, a significant improvement in diaphragm height was observed at 22 h after IAVI treatment. The wet-to-dry weight ratio of the lungs in IAVI group was also significantly higher than that that in the sham-operated control group. CONCLUSION: Our data indicate that IAVI surgery could improve the damaged pulmonary function caused by IAH after hemorrhagic shock resuscitation (Tab. 1, Fig. 7, Ref. 21).
RCT Entities:
PURPOSE: Intra-abdominal hypertension (IAH) causes lung dysfunction in patients after hemorrhagic shock resuscitation. We performed intra-abdominal volume increment (IAVI) to IAH and studied its effect on pulmonary function. METHODS: Eight minipigs established for IAH model by exsanquination until shock, incomplete blockage of portal vein and overload resuscitation were randomly divided into IAVI group (n=4) and sham-operated control group (n=4). Bladder pressure, arterial blood gas analysis and thoraco-abdominal computed tomography (CT) scans were measured. The minipigs were sacrificed 26 h after surgery, and lung samples were harvested for measuring the wet-to-dry weight ratio and hematoxylin-eosin staining. RESULTS: Compared with sham-operated control group, the respiratory rate and paCO2 remarkably decreased and paO2 notably increased at 8 and 12 h in the IAVI group. The bladder pressure also notably decreased at 8, 12, and 22 h after IAVI treatment. However, a significant improvement in diaphragm height was observed at 22 h after IAVI treatment. The wet-to-dry weight ratio of the lungs in IAVI group was also significantly higher than that that in the sham-operated control group. CONCLUSION: Our data indicate that IAVI surgery could improve the damaged pulmonary function caused by IAH after hemorrhagic shock resuscitation (Tab. 1, Fig. 7, Ref. 21).