BACKGROUND: Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. RESULTS/ METHODOLOGY: A stable isotope dilution LC-MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1-160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze-thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers. CONCLUSION: These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.
BACKGROUND:Desmosine/isodesmosine (DES/IDS) is a promising biomarker for estimating activity of elastin degradation. RESULTS/ METHODOLOGY: A stable isotope dilution LC-MS/MS method for measuring serum/plasma DES/IDS was developed and validated. The reportable range of this assay was 0.1-160 ng/ml. Serum/plasma DES/IDS level was stable at room temperature or 4°C for 20 h, and for three freeze-thaw cycles. Interferences from endogenous compounds and ion suppression/enhancing effect were also evaluated. Our results suggest the absolute necessity of using an IS in the measurement. We found that serum/plasma DES/IDS levels from patients with chronic obstructive pulmonary disease and cystic fibrosis were significantly higher compared with healthy smokers. CONCLUSION: These results demonstrate that the LC-MS/MS method provides sensitive, reproducible and accurate quantification of total serum/plasma DES/IDS.
Authors: John R Hurst; Joanne Beckmann; Yanyan Ni; Charlotte E Bolton; Carmel M McEniery; John R Cockcroft; Neil Marlow Journal: Am J Respir Crit Care Med Date: 2020-08-01 Impact factor: 21.405
Authors: Andrea Gramegna; Francesco Amati; Leonardo Terranova; Giovanni Sotgiu; Paolo Tarsia; Daniela Miglietta; Maria Adelaide Calderazzo; Stefano Aliberti; Francesco Blasi Journal: Respir Res Date: 2017-12-19
Authors: Ify R Mordi; Rachael O Forsythe; Corry Gellatly; Zaid Iskandar; Olivia M McBride; Athanasios Saratzis; Rod Chalmers; Calvin Chin; Matthew J Bown; David E Newby; Chim C Lang; Jeffrey T J Huang; Anna-Maria Choy Journal: J Am Heart Assoc Date: 2019-10-09 Impact factor: 5.501
Authors: James D Chalmers; Kelly L Moffitt; Guillermo Suarez-Cuartin; Oriol Sibila; Simon Finch; Elizabeth Furrie; Alison Dicker; Karolina Wrobel; J Stuart Elborn; Brian Walker; S Lorraine Martin; Sara E Marshall; Jeffrey T-J Huang; Thomas C Fardon Journal: Am J Respir Crit Care Med Date: 2017-05-15 Impact factor: 21.405
Authors: Bart de Brouwer; Marjolein Drent; Jody M W van den Ouweland; Petal A Wijnen; Coline H M van Moorsel; Otto Bekers; Jan C Grutters; Eric S White; Rob Janssen Journal: Respir Res Date: 2018-03-20