| Literature DB >> 23933420 |
Qiurong Zhao1, Fei Liu, Shun Chen, Xiaoling Yan, Yulin Qi, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xiaoyue Chen, Anchun Cheng.
Abstract
CD8 molecule is a cell membrane glycoprotein, which plays an important role in cell-mediated immunity. Here, we identified Chinese goose CD8α (goCD8α) gene for the first time. The full-length cDNA of goCD8α is 1459bp in length and contains a 711bp open reading frame. Phylogenetic analysis shows that the waterfowl CD8α formed a monophyletic group. Semi-quantitative RT-PCR analysis showed that transcripts of goCD8α mRNA were high in the immune-related organs and mucosal immune system in gosling, and high in thymus and spleen comparing to other immune-related tissues in goose. The obvious increase of CD8α expression was observed in spleen of acute new type gosling viral enteritis virus (NGVEV) infected bird, while the increase of CD8α were observed in the thymus, bursa of fabricius, and cecum of chronic infected bird. The CD8α mRNA transcription level in spleen mononuclear cells was significantly up-regulated when stimulated by phytohemagglutinin, but not by lipopolysaccharide in vitro.Entities:
Keywords: AAP; AP; AUAP; Adapter Primer; BF; BLAST; Basic Local Alignment Search Tool; CD8α; CHCD8α; CIS; CMC; CMI; CT; CTLs; GSP; Goose; HG; IEL; Immunobiological activity; LD(50); LPS; MHC; MIS; MNCs; Molecular cloning; NGVEV; NGVEV infection; OFR; PHA; PI; RACE; RT-PCR; T cell antigen receptor; TCID50; TCR; Tissue distribution; TuCD8α; UTR; abridged anchor primer; abridged universal amplification primer; bursa of fabricius; cDNA; cecal tonsil; cell-mediated cytotoxicity; cell-mediated immune; chicken CD8α; complementary to RNA; cutaneous immune system; cytotoxic T lymphocytes; gene specific primer; goCD8α; goose CD8α; harderian gland; intestinal intraepithelial lymphocytes; lethal dose; lipopolysaccharide; major histocompatibility complex; monocolor cells; mucosal immune system; new type gosling viral enteritis virus; open reading frame; phytohemagglutinin; post infection; qPCR; quantitative PCR; rapid amplification of cDNA ends; reverse transcription-polymerase chain reaction; tissue culture infective dose; turkey CD8α; untranslated region
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Year: 2013 PMID： 23933420 DOI： 10.1016/j.gene.2013.07.104
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688