Genome Sequence of Staphylococcus massiliensis Strain S46, Isolated from the Surface of Healthy Human Skin.

Staphylococcus massiliensis strain S46 was isolated from the surface of healthy human skin. Here, we report the draft genome sequence of S. massiliensis S46 (2,447,110 bp, with a G+C content of 36.3%).

GENOME ANNOUNCEMENT

Staphylococcus massiliensis strain S46 was isolated from the surface of healthy human skin and was identified as S. massiliensis based on the rrs (16S rRNA) gene sequence identity of 100% with type strain S. massiliensis 5402776 T. S. massiliensis is a coagulase-negative, nonmotile, non-spore-forming bacterium that was first isolated from a human brain abscess (1) and later was proposed as a component of normal human skin microflora (2). The genome sequence of strain 5402776 T was reported recently (3). Here, we report the draft genome sequence of S. massiliensis S46.

The genomic DNA of S. massiliensis S46 was extracted from culture grown on Luria-Bertani (LB) broth. The genome of S. massiliensis S46 was sequenced by a whole-genome shotgun sequencing strategy using 454 GS FLX, which produced a total of 157,855 reads. The reads were assembled into 64 large (>500 bp) contigs by Newbler (version 2.6). The contigs were annotated using the prokaryotic genome automatic annotation pipeline at NCBI. The automated pipeline uses GeneMark and Glimmer (4, 5, 6) for gene prediction and compares the translated proteins with the nonredundant protein database at GenBank, Entrez Protein Clusters (7), the Conserved Domain Database (8), and COGs (9) for annotation. The genome sequence was also submitted to the RAST (10) server for annotation and identification of metabolic pathways.

The S. massiliensis S46 unclosed draft genome is 2,447,110 bp in length, with a G+C content of 36.3%. The genome contains 2,324 coding sequences, 56 tRNA genes, and 6 rRNA genes. The strain S46 genome has predicted genes for glycolysis, the tricarboxylic acid (TCA) cycle, fatty acid metabolism, phosphorus metabolism, iron acquisition, and organic sulfur assimilation.

A comparison with the genome sequences available at RAST showed that Staphylococcus epidermidis strain RP62A (score, 523) was the closest neighbor of S. massiliensis S46. The genes for N-linked glycosylation, sialic acid metabolism, thiamin biosynthesis, and trehalose uptake and utilization were present in S. massiliensis S46 but not in S. epidermidis RP62A. However, the genes for biofilm formation, quorum sensing, late competence, arginine biosynthesis, inorganic sulfur assimilation, and urea decomposition were absent in S. massiliensis S46 but were present in S. epidermidis RP62A. A detailed comparative genome analysis between the strains of S. massiliensis and S. epidermidis will reveal their metabolic differences and strategies to survive as commensals on the human skin surface.

Nucleotide sequence accession numbers.

This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession no. AMSQ00000000. The version described in this paper is the first version, AMSQ01000000. The 64 contigs have been deposited under the accession no. AMSQ01000001 to AMSQ01000064.