| Literature DB >> 23929105 |
Abstract
Transgenic expression of catalase in mitochondria using a transgenic strategy extends life span and prevents aging-related pathology in mice. However, transgenic overexpression is not suitable for a clinical application. Adeno-associated virus (AAV) is the most promising gene delivery vehicle. Here we outline strategies on the generation of an AAV vector expressing the mitochondria-targeted catalase gene (AV.RSV.MCAT). We also describe methods for evaluating physiological impact of AV.RSV.MCAT on muscle contractility and running performance in mice.Entities:
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Year: 2013 PMID： 23929105 PMCID： PMC4539949 DOI： 10.1007/978-1-62703-556-9_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745